Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching

David Holmes, Graeme Whyte, Andrew Ekpenyong, Jochen Guck, Thomas Duke

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work we present data showing deformability-based cell separation in a deterministic lateral displacement (DLD) device. We use cells of defined stiffness (glutaraldehyde cross-linked erythrocytes) to test the performance of the device across a range of cell stiffness and applied shear rates. Optical stretching is used as an independent method of quantifying the stiffness of the cells, thus allowing better understanding of the DLD system for deformability based cell separation. Displacement is shown to correlate with cell stiffness as measured across a range of flow rates. Data showing how the isolation of leukocytes from whole blood varies with shear rate is also presented.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages536-538
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Fingerprint

Formability
Stretching
Stiffness
Chemical analysis
Shear deformation
Glutaral
Blood
Flow rate

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Holmes, D., Whyte, G., Ekpenyong, A., Guck, J., & Duke, T. (2012). Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 536-538). Chemical and Biological Microsystems Society.

Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching. / Holmes, David; Whyte, Graeme; Ekpenyong, Andrew; Guck, Jochen; Duke, Thomas.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 536-538.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Holmes, D, Whyte, G, Ekpenyong, A, Guck, J & Duke, T 2012, Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 536-538, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
Holmes D, Whyte G, Ekpenyong A, Guck J, Duke T. Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 536-538
Holmes, David ; Whyte, Graeme ; Ekpenyong, Andrew ; Guck, Jochen ; Duke, Thomas. / Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 536-538
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