Abstract
In this work we present data showing deformability-based cell separation in a deterministic lateral displacement (DLD) device. We use cells of defined stiffness (glutaraldehyde cross-linked erythrocytes) to test the performance of the device across a range of cell stiffness and applied shear rates. Optical stretching is used as an independent method of quantifying the stiffness of the cells, thus allowing better understanding of the DLD system for deformability based cell separation. Displacement is shown to correlate with cell stiffness as measured across a range of flow rates. Data showing how the isolation of leukocytes from whole blood varies with shear rate is also presented.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 536-538 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| State | Published - 2012 |
| Externally published | Yes |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: Oct 28 2012 → Nov 1 2012 |
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country | Japan |
| City | Okinawa |
| Period | 10/28/12 → 11/1/12 |
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All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching. / Holmes, David; Whyte, Graeme; Ekpenyong, Andrew; Guck, Jochen; Duke, Thomas.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 536-538.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching
AU - Holmes, David
AU - Whyte, Graeme
AU - Ekpenyong, Andrew
AU - Guck, Jochen
AU - Duke, Thomas
PY - 2012
Y1 - 2012
N2 - In this work we present data showing deformability-based cell separation in a deterministic lateral displacement (DLD) device. We use cells of defined stiffness (glutaraldehyde cross-linked erythrocytes) to test the performance of the device across a range of cell stiffness and applied shear rates. Optical stretching is used as an independent method of quantifying the stiffness of the cells, thus allowing better understanding of the DLD system for deformability based cell separation. Displacement is shown to correlate with cell stiffness as measured across a range of flow rates. Data showing how the isolation of leukocytes from whole blood varies with shear rate is also presented.
AB - In this work we present data showing deformability-based cell separation in a deterministic lateral displacement (DLD) device. We use cells of defined stiffness (glutaraldehyde cross-linked erythrocytes) to test the performance of the device across a range of cell stiffness and applied shear rates. Optical stretching is used as an independent method of quantifying the stiffness of the cells, thus allowing better understanding of the DLD system for deformability based cell separation. Displacement is shown to correlate with cell stiffness as measured across a range of flow rates. Data showing how the isolation of leukocytes from whole blood varies with shear rate is also presented.
UR - http://www.scopus.com/inward/record.url?scp=84901801238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901801238&partnerID=8YFLogxK
M3 - Conference contribution
SN - 9780979806452
SP - 536
EP - 538
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
ER -