Ranakinin

A novel NK1 tachykinin receptor agonist isolated with neurokinin B from the brain of the frog Rana ridibunda

Finbarr O'Harte, Elizabeth Burcher, Sándor Lovas, D. David Smith, Hubert Vaudry, J. Michael Conlon

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

An extract of the whole brain of the frog Rana ridibunda contained high concentrations of substance P-like immunoreactivity, measured with an antiserum directed against the COOH-terminal region of mammalian substance P and neurokinin B-like immunoreactivity, measured with an antiserum directed against the NH2-terminus of neurokinin B. The primary structure of the substance P-related peptide (ranakinin) was established as: Lys-Pro-Asn-Pro-GluArg-Phe-Tyr-Gly-Leu-Met-NH2. Mammalian substance P was not present in the extract. The primary structure of the neurokinin B-related peptide was established as: Asp-Met-His-Asp-Phe-Phe-Val-Gly-Leu-Met-NH2. This amino acid sequence is the same as that of mammalian neurokinin B. Ranakinin was equipotent with substance P and [Sar9,-Met(O2)11]substance P in inhibiting the binding of 125I-Bolton-Hunter-[Sar9,Met(O2) 11]substance P, a selective radioligand for the NK1 receptor, to binding sites in rat submandibular gland membranes (IC50 1.6 ± 0.3 nM; n = 5). It is concluded that ranakinin is a preferred agonist for the mammalian NK1 tachykinin receptor subtype.

Original languageEnglish
Pages (from-to)2086-2091
Number of pages6
JournalJournal of Neurochemistry
Volume57
Issue number6
StatePublished - Dec 1991

Fingerprint

Rana ridibunda
Neurokinin B
Tachykinin Receptors
Substance P
Anura
Brain
Immune Sera
Peptides
Submandibular Gland
ranakinin
Inhibitory Concentration 50
Rats
Amino Acid Sequence
Binding Sites
Membranes
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Ranakinin : A novel NK1 tachykinin receptor agonist isolated with neurokinin B from the brain of the frog Rana ridibunda. / O'Harte, Finbarr; Burcher, Elizabeth; Lovas, Sándor; Smith, D. David; Vaudry, Hubert; Conlon, J. Michael.

In: Journal of Neurochemistry, Vol. 57, No. 6, 12.1991, p. 2086-2091.

Research output: Contribution to journalArticle

O'Harte, Finbarr ; Burcher, Elizabeth ; Lovas, Sándor ; Smith, D. David ; Vaudry, Hubert ; Conlon, J. Michael. / Ranakinin : A novel NK1 tachykinin receptor agonist isolated with neurokinin B from the brain of the frog Rana ridibunda. In: Journal of Neurochemistry. 1991 ; Vol. 57, No. 6. pp. 2086-2091.
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