Rapid field inversion gel electrophoresis in combination with an rRNA gene probe in the epidemiological evaluation of staphylococci

Richard V. Goering, T. D. Duensing

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

A rapid field inversion gel electrophoresis (FIGE) protocol was combined with an rRNA gene probe in the analysis of staphylococci that were difficult to study epidemiologically by conventional means. The following groups of clinical isolates were examined: (i) predominantly antibiotic-susceptible Staphylococcus aureus strains containing no detectable plasmids and unresponsive to bacteriophage typing and (ii) methicillin-resistant Staphylococcus epidermidis strains carrying a single plasmid ca. 30 kilobase in size. The results indicated that strain interrelationships could be established on the basis of SmaI-generated chromosomal restriction fragment length polymorphisms (RFLPs) analyzed by FIGE. RFLP analysis of strains known to be unrelated established the importance of minor differences in DNA banding patterns as indicators of strain dissimilarities. Hybridization studies with an rRNA gene probe confirmed this conclusion. These results suggest that FIGE analysis of chromosomal RFLPs (especially in combination with molecular probes) is an important addition to the armamentarium of molecular epidemiology.

Original languageEnglish
Pages (from-to)426-429
Number of pages4
JournalJournal of Clinical Microbiology
Volume28
Issue number3
StatePublished - 1990

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Pulsed Field Gel Electrophoresis
Staphylococcus
rRNA Genes
Restriction Fragment Length Polymorphisms
Plasmids
Bacteriophage Typing
Molecular Probes
Methicillin Resistance
Staphylococcus epidermidis
Molecular Epidemiology
Staphylococcus aureus
Anti-Bacterial Agents
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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