Rapid, high-throughput detection of PrPSc by 96-well immunoassay

Michelle L. Kramer, Jason C. Bartz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Prion diseases are emerging infectious disorders that affect several mammalian species including humans. The transmissible agent is comprised of PrPSc, a misfolded isoform of the normal host-encoded prion protein PrPC. Immunodetection of PrPSc is often utilized for prion disease diagnosis and tracking spread of the infectious agent through the host. We have developed a rapid, high-throughput 96-well immunoassay, which is specific for the detection of PrPSc. This assay has PrPSc detection limits similar to western blot and is advantageous because of its comparatively shorter running time, smaller start-up and operation costs and large sample capacity.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalPrion
Volume3
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Prion Diseases
Prions
Immunoassay
PrPC Proteins
Throughput
Running
Limit of Detection
Assays
Protein Isoforms
Western Blotting
Costs and Cost Analysis
Costs
Prion Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Infectious Diseases
  • Cellular and Molecular Neuroscience

Cite this

Rapid, high-throughput detection of PrPSc by 96-well immunoassay. / Kramer, Michelle L.; Bartz, Jason C.

In: Prion, Vol. 3, No. 1, 01.2009, p. 44-48.

Research output: Contribution to journalArticle

Kramer, Michelle L. ; Bartz, Jason C. / Rapid, high-throughput detection of PrPSc by 96-well immunoassay. In: Prion. 2009 ; Vol. 3, No. 1. pp. 44-48.
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