Rapid, high-throughput detection of PrPSc by 96-well immunoassay

Michelle L. Kramer; Jason C. Bartz

doi:10.4161/pri.3.1.8442

Rapid, high-throughput detection of PrP^Sc by 96-well immunoassay

Michelle L. Kramer, Jason C. Bartz

Department of Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Prion diseases are emerging infectious disorders that affect several mammalian species including humans. The transmissible agent is comprised of PrP^Sc, a misfolded isoform of the normal host-encoded prion protein PrP^C. Immunodetection of PrP^Sc is often utilized for prion disease diagnosis and tracking spread of the infectious agent through the host. We have developed a rapid, high-throughput 96-well immunoassay, which is specific for the detection of PrP^Sc. This assay has PrP^Sc detection limits similar to western blot and is advantageous because of its comparatively shorter running time, smaller start-up and operation costs and large sample capacity.

Original language	English (US)
Pages (from-to)	44-48
Number of pages	5
Journal	Prion
Volume	3
Issue number	1
DOIs	https://doi.org/10.4161/pri.3.1.8442
State	Published - Jan 2009

All Science Journal Classification (ASJC) codes

Biochemistry
Cellular and Molecular Neuroscience
Cell Biology
Infectious Diseases

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title = "Rapid, high-throughput detection of PrPSc by 96-well immunoassay",

abstract = "Prion diseases are emerging infectious disorders that affect several mammalian species including humans. The transmissible agent is comprised of PrPSc, a misfolded isoform of the normal host-encoded prion protein PrPC. Immunodetection of PrPSc is often utilized for prion disease diagnosis and tracking spread of the infectious agent through the host. We have developed a rapid, high-throughput 96-well immunoassay, which is specific for the detection of PrPSc. This assay has PrPSc detection limits similar to western blot and is advantageous because of its comparatively shorter running time, smaller start-up and operation costs and large sample capacity.",

author = "Kramer, {Michelle L.} and Bartz, {Jason C.}",

note = "Funding Information: This work was supported by the National Center for Research Resources (P20 RR0115635-6 and C06 RR17417-01), the National Institute for Neurological Disorders and Stroke (R01 NS052609) and the National Prion Research Program (NP020041).",

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doi = "10.4161/pri.3.1.8442",

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AU - Kramer, Michelle L.

AU - Bartz, Jason C.

N1 - Funding Information: This work was supported by the National Center for Research Resources (P20 RR0115635-6 and C06 RR17417-01), the National Institute for Neurological Disorders and Stroke (R01 NS052609) and the National Prion Research Program (NP020041).

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N2 - Prion diseases are emerging infectious disorders that affect several mammalian species including humans. The transmissible agent is comprised of PrPSc, a misfolded isoform of the normal host-encoded prion protein PrPC. Immunodetection of PrPSc is often utilized for prion disease diagnosis and tracking spread of the infectious agent through the host. We have developed a rapid, high-throughput 96-well immunoassay, which is specific for the detection of PrPSc. This assay has PrPSc detection limits similar to western blot and is advantageous because of its comparatively shorter running time, smaller start-up and operation costs and large sample capacity.

AB - Prion diseases are emerging infectious disorders that affect several mammalian species including humans. The transmissible agent is comprised of PrPSc, a misfolded isoform of the normal host-encoded prion protein PrPC. Immunodetection of PrPSc is often utilized for prion disease diagnosis and tracking spread of the infectious agent through the host. We have developed a rapid, high-throughput 96-well immunoassay, which is specific for the detection of PrPSc. This assay has PrPSc detection limits similar to western blot and is advantageous because of its comparatively shorter running time, smaller start-up and operation costs and large sample capacity.

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Rapid, high-throughput detection of PrP^Sc by 96-well immunoassay

Abstract

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