Rapid, high-throughput detection of PrPSc by 96-well immunoassay

Michelle L. Kramer, Jason C. Bartz

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Prion diseases are emerging infectious disorders that affect several mammalian species including humans. The transmissible agent is comprised of PrPSc, a misfolded isoform of the normal host-encoded prion protein PrPC. Immunodetection of PrPSc is often utilized for prion disease diagnosis and tracking spread of the infectious agent through the host. We have developed a rapid, high-throughput 96-well immunoassay, which is specific for the detection of PrPSc. This assay has PrPSc detection limits similar to western blot and is advantageous because of its comparatively shorter running time, smaller start-up and operation costs and large sample capacity.

Original languageEnglish (US)
Pages (from-to)44-48
Number of pages5
JournalPrion
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2009

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Infectious Diseases

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