Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis

Richard V. Goering, M. A. Winters

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

We report a rapid method for the isolation of intact chromosomal DNA from gram-positive cocci that is suitable for in situ restriction endonuclease digestion in agarose blocks. When combined with a rapid field inversion gel electrophoresis protocol, this approach allows the preparation and electrophoretic analysis of chromosomal restriction fragments produced by rare-cutting enzymes in a total time period of 2 days from start to finish. The utility of the method is demonstrated in the epidemiological evaluation of Staphylococcus epidermidis clusters from two hospitals as well as of additional representative staphylococci and enterococci.

Original languageEnglish
Pages (from-to)577-580
Number of pages4
JournalJournal of Clinical Microbiology
Volume30
Issue number3
StatePublished - 1992

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Epidemiologic Methods
Gram-Positive Cocci
Pulsed Field Gel Electrophoresis
Staphylococcus epidermidis
DNA Restriction Enzymes
Enterococcus
Staphylococcus
Sepharose
Digestion
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis. / Goering, Richard V.; Winters, M. A.

In: Journal of Clinical Microbiology, Vol. 30, No. 3, 1992, p. 577-580.

Research output: Contribution to journalArticle

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