Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N. Geyer; Nancy D. Hanson

doi:10.1016/j.diagmicrobio.2013.06.015

Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N. Geyer, Nancy D. Hanson

Department of Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

Original language	English (US)
Pages (from-to)	113-117
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	77
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2013.06.015
State	Published - Oct 2013

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2013.06.015

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title = "Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens",

abstract = "A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.",

author = "Geyer, {Chelsie N.} and Hanson, {Nancy D.}",

note = "Funding Information: We would like to thank Dr. Mark Reisbig for the generation of the pmAmpC E. coli K12 transformant controls. This study was supported by a grant from Streck Inc. ",

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AU - Hanson, Nancy D.

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AB - A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

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Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Abstract

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