Abstract
A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.
| Original language | English |
|---|---|
| Pages (from-to) | 113-117 |
| Number of pages | 5 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Volume | 77 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 2013 |
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All Science Journal Classification (ASJC) codes
- Microbiology (medical)
- Infectious Diseases
Cite this
Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens. / Geyer, Chelsie N.; Hanson, Nancy D.
In: Diagnostic Microbiology and Infectious Disease, Vol. 77, No. 2, 10.2013, p. 113-117.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens
AU - Geyer, Chelsie N.
AU - Hanson, Nancy D.
PY - 2013/10
Y1 - 2013/10
N2 - A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.
AB - A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.
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UR - http://www.scopus.com/inward/citedby.url?scp=84884416352&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2013.06.015
DO - 10.1016/j.diagmicrobio.2013.06.015
M3 - Article
C2 - 23891223
AN - SCOPUS:84884416352
VL - 77
SP - 113
EP - 117
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 2
ER -