Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N. Geyer, Nancy D. Hanson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume77
Issue number2
DOIs
StatePublished - Oct 2013

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Microbial Drug Resistance
Polymerase Chain Reaction
Genes
Multiplex Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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