Abstract
A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.
Original language | English (US) |
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Pages (from-to) | 113-117 |
Number of pages | 5 |
Journal | Diagnostic Microbiology and Infectious Disease |
Volume | 77 |
Issue number | 2 |
DOIs | |
State | Published - Oct 2013 |
All Science Journal Classification (ASJC) codes
- Microbiology (medical)
- Infectious Diseases