Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N. Geyer; Nancy D. Hanson

doi:10.1016/j.diagmicrobio.2013.06.015

Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N. Geyer, Nancy D. Hanson

Department of Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

Original language	English (US)
Pages (from-to)	113-117
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	77
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2013.06.015
State	Published - Oct 1 2013

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

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abstract = "A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.",

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