A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.
|Original language||English (US)|
|Number of pages||5|
|Journal||Diagnostic Microbiology and Infectious Disease|
|State||Published - Oct 1 2013|
All Science Journal Classification (ASJC) codes
- Microbiology (medical)
- Infectious Diseases