Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N. Geyer; Nancy D. Hanson

doi:10.1016/j.diagmicrobio.2013.06.015

Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Chelsie N. Geyer, Nancy D. Hanson

Department of Medical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

Original language	English (US)
Pages (from-to)	113-117
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	77
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2013.06.015
State	Published - Oct 2013

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2013.06.015

Cite this

@article{7482c4a47aed40349b26a93f2c45e2e3,

title = "Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens",

abstract = "A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.",

author = "Geyer, {Chelsie N.} and Hanson, {Nancy D.}",

note = "Funding Information: We would like to thank Dr. Mark Reisbig for the generation of the pmAmpC E. coli K12 transformant controls. This study was supported by a grant from Streck Inc. ",

year = "2013",

month = oct,

doi = "10.1016/j.diagmicrobio.2013.06.015",

language = "English (US)",

volume = "77",

pages = "113--117",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

AU - Geyer, Chelsie N.

AU - Hanson, Nancy D.

N1 - Funding Information: We would like to thank Dr. Mark Reisbig for the generation of the pmAmpC E. coli K12 transformant controls. This study was supported by a grant from Streck Inc.

PY - 2013/10

Y1 - 2013/10

N2 - A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

AB - A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16-18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms.

UR - http://www.scopus.com/inward/record.url?scp=84884416352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884416352&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2013.06.015

DO - 10.1016/j.diagmicrobio.2013.06.015

M3 - Article

C2 - 23891223

AN - SCOPUS:84884416352

VL - 77

SP - 113

EP - 117

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 2

ER -

Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this