Real-time deformability cytometry: On-the-fly cell mechanical phenotyping

Oliver Otto; Philipp Rosendahl; Alexander Mietke; Stefan Golfier; Christoph Herold; Daniel Klaue; Salvatore Girardo; Stefano Pagliara; Andrew Ekpenyong; Angela Jacobi; Manja Wobus; Nicole Töpfner; Ulrich F. Keyser; Jörg Mansfeld; Elisabeth Fischer-Friedrich; Jochen Guck

doi:10.1038/nmeth.3281

Real-time deformability cytometry: On-the-fly cell mechanical phenotyping

Oliver Otto, Philipp Rosendahl, Alexander Mietke, Stefan Golfier, Christoph Herold, Daniel Klaue, Salvatore Girardo, Stefano Pagliara, Andrew Ekpenyong, Angela Jacobi, Manja Wobus, Nicole Töpfner, Ulrich F. Keyser, Jörg Mansfeld, Elisabeth Fischer-Friedrich, Jochen Guck

Department of Physics

Research output: Contribution to journal › Article

249 Scopus citations

Abstract

We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

Original language	English (US)
Pages (from-to)	199-202
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.3281
State	Published - Feb 26 2015

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All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Cite this

Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D., Girardo, S., Pagliara, S., Ekpenyong, A., Jacobi, A., Wobus, M., Töpfner, N., Keyser, U. F., Mansfeld, J., Fischer-Friedrich, E., & Guck, J. (2015). Real-time deformability cytometry: On-the-fly cell mechanical phenotyping. Nature Methods, 12(3), 199-202. https://doi.org/10.1038/nmeth.3281

Real-time deformability cytometry : On-the-fly cell mechanical phenotyping. / Otto, Oliver; Rosendahl, Philipp; Mietke, Alexander; Golfier, Stefan; Herold, Christoph; Klaue, Daniel; Girardo, Salvatore; Pagliara, Stefano; Ekpenyong, Andrew; Jacobi, Angela; Wobus, Manja; Töpfner, Nicole; Keyser, Ulrich F.; Mansfeld, Jörg; Fischer-Friedrich, Elisabeth; Guck, Jochen.

In: Nature Methods, Vol. 12, No. 3, 26.02.2015, p. 199-202.

Research output: Contribution to journal › Article

Otto, Oliver ; Rosendahl, Philipp ; Mietke, Alexander ; Golfier, Stefan ; Herold, Christoph ; Klaue, Daniel ; Girardo, Salvatore ; Pagliara, Stefano ; Ekpenyong, Andrew ; Jacobi, Angela ; Wobus, Manja ; Töpfner, Nicole ; Keyser, Ulrich F. ; Mansfeld, Jörg ; Fischer-Friedrich, Elisabeth ; Guck, Jochen. / Real-time deformability cytometry : On-the-fly cell mechanical phenotyping. In: Nature Methods. 2015 ; Vol. 12, No. 3. pp. 199-202.

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10.1038/nmeth.3281