@article{8aae9f69bef446618b7160a0c9208c72,
title = "Real-time deformability cytometry: On-the-fly cell mechanical phenotyping",
abstract = "We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.",
author = "Oliver Otto and Philipp Rosendahl and Alexander Mietke and Stefan Golfier and Christoph Herold and Daniel Klaue and Salvatore Girardo and Stefano Pagliara and Andrew Ekpenyong and Angela Jacobi and Manja Wobus and Nicole T{\"o}pfner and Keyser, {Ulrich F.} and J{\"o}rg Mansfeld and Elisabeth Fischer-Friedrich and Jochen Guck",
note = "Funding Information: We thank A. Taubenberger, M. Herbig, C. Liebers, L. Menschner, C. Klug, R. Berner, M. Bornh{\"a}user, C. Bryant, E. Chilvers, B. Friedrich, A. Voigt, J. Tegenfeldt, M. Tsch{\"o}p, F. Amblard, T. Hyman and S. Grill for technical support, advice and engaging discussions. The HL60/S4 cells were a generous gift of D. and A. Olins (University of New England). Financial support from the Alexander-von-Humboldt Stiftung (Humboldt-Professorship to J.G.), S{\"a}chsisches Ministerium f{\"u}r Wissenschaft und Kunst (TG70 grant to O.O. and J.G.), DFG-Center for Regenerative Medicine of the Technische Universit{\"a}t Dresden (seed grant to J.G.), Deutsche Forschungsgemeinschaft (DFG Emmy Noether Grant to J.M., MA 5831/1-1; KFO249 Gerok position to N.T.), Deutsche Gesellschaft f{\"u}r P{\"a}diatrische Infektiologie (N.T.) and Studienstiftung des Deutschen Volkes (A.M.), Max Planck Society (E.F.-F.) and Leverhulme and Newton Trust (Early Career Fellowship to S.P.) is gratefully acknowledged.",
year = "2015",
month = feb,
day = "26",
doi = "10.1038/nmeth.3281",
language = "English (US)",
volume = "12",
pages = "199--202",
journal = "Nature Methods",
issn = "1548-7091",
number = "3",
}