Real-time deformability cytometry

On-the-fly cell mechanical phenotyping

Oliver Otto, Philipp Rosendahl, Alexander Mietke, Stefan Golfier, Christoph Herold, Daniel Klaue, Salvatore Girardo, Stefano Pagliara, Andrew Ekpenyong, Angela Jacobi, Manja Wobus, Nicole Töpfner, Ulrich F. Keyser, Jörg Mansfeld, Elisabeth Fischer-Friedrich, Jochen Guck

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalNature Methods
Volume12
Issue number3
DOIs
StatePublished - Feb 26 2015

Fingerprint

Formability
Cells
Flow cytometry
Biotechnology
Stem cells
Medicine
Blood
Dermatoglyphics
Population
Cell Differentiation
Cell Cycle
Flow Cytometry
Stem Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D., ... Guck, J. (2015). Real-time deformability cytometry: On-the-fly cell mechanical phenotyping. Nature Methods, 12(3), 199-202. https://doi.org/10.1038/nmeth.3281

Real-time deformability cytometry : On-the-fly cell mechanical phenotyping. / Otto, Oliver; Rosendahl, Philipp; Mietke, Alexander; Golfier, Stefan; Herold, Christoph; Klaue, Daniel; Girardo, Salvatore; Pagliara, Stefano; Ekpenyong, Andrew; Jacobi, Angela; Wobus, Manja; Töpfner, Nicole; Keyser, Ulrich F.; Mansfeld, Jörg; Fischer-Friedrich, Elisabeth; Guck, Jochen.

In: Nature Methods, Vol. 12, No. 3, 26.02.2015, p. 199-202.

Research output: Contribution to journalArticle

Otto, O, Rosendahl, P, Mietke, A, Golfier, S, Herold, C, Klaue, D, Girardo, S, Pagliara, S, Ekpenyong, A, Jacobi, A, Wobus, M, Töpfner, N, Keyser, UF, Mansfeld, J, Fischer-Friedrich, E & Guck, J 2015, 'Real-time deformability cytometry: On-the-fly cell mechanical phenotyping', Nature Methods, vol. 12, no. 3, pp. 199-202. https://doi.org/10.1038/nmeth.3281
Otto O, Rosendahl P, Mietke A, Golfier S, Herold C, Klaue D et al. Real-time deformability cytometry: On-the-fly cell mechanical phenotyping. Nature Methods. 2015 Feb 26;12(3):199-202. https://doi.org/10.1038/nmeth.3281
Otto, Oliver ; Rosendahl, Philipp ; Mietke, Alexander ; Golfier, Stefan ; Herold, Christoph ; Klaue, Daniel ; Girardo, Salvatore ; Pagliara, Stefano ; Ekpenyong, Andrew ; Jacobi, Angela ; Wobus, Manja ; Töpfner, Nicole ; Keyser, Ulrich F. ; Mansfeld, Jörg ; Fischer-Friedrich, Elisabeth ; Guck, Jochen. / Real-time deformability cytometry : On-the-fly cell mechanical phenotyping. In: Nature Methods. 2015 ; Vol. 12, No. 3. pp. 199-202.
@article{8aae9f69bef446618b7160a0c9208c72,
title = "Real-time deformability cytometry: On-the-fly cell mechanical phenotyping",
abstract = "We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.",
author = "Oliver Otto and Philipp Rosendahl and Alexander Mietke and Stefan Golfier and Christoph Herold and Daniel Klaue and Salvatore Girardo and Stefano Pagliara and Andrew Ekpenyong and Angela Jacobi and Manja Wobus and Nicole T{\"o}pfner and Keyser, {Ulrich F.} and J{\"o}rg Mansfeld and Elisabeth Fischer-Friedrich and Jochen Guck",
year = "2015",
month = "2",
day = "26",
doi = "10.1038/nmeth.3281",
language = "English",
volume = "12",
pages = "199--202",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Real-time deformability cytometry

T2 - On-the-fly cell mechanical phenotyping

AU - Otto, Oliver

AU - Rosendahl, Philipp

AU - Mietke, Alexander

AU - Golfier, Stefan

AU - Herold, Christoph

AU - Klaue, Daniel

AU - Girardo, Salvatore

AU - Pagliara, Stefano

AU - Ekpenyong, Andrew

AU - Jacobi, Angela

AU - Wobus, Manja

AU - Töpfner, Nicole

AU - Keyser, Ulrich F.

AU - Mansfeld, Jörg

AU - Fischer-Friedrich, Elisabeth

AU - Guck, Jochen

PY - 2015/2/26

Y1 - 2015/2/26

N2 - We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

AB - We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

UR - http://www.scopus.com/inward/record.url?scp=84923846469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923846469&partnerID=8YFLogxK

U2 - 10.1038/nmeth.3281

DO - 10.1038/nmeth.3281

M3 - Article

VL - 12

SP - 199

EP - 202

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 3

ER -