Abstract
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
| Original language | English |
|---|---|
| Pages (from-to) | 199-202 |
| Number of pages | 4 |
| Journal | Nature Methods |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 26 2015 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Molecular Biology
- Biochemistry
- Cell Biology
Cite this
Real-time deformability cytometry : On-the-fly cell mechanical phenotyping. / Otto, Oliver; Rosendahl, Philipp; Mietke, Alexander; Golfier, Stefan; Herold, Christoph; Klaue, Daniel; Girardo, Salvatore; Pagliara, Stefano; Ekpenyong, Andrew; Jacobi, Angela; Wobus, Manja; Töpfner, Nicole; Keyser, Ulrich F.; Mansfeld, Jörg; Fischer-Friedrich, Elisabeth; Guck, Jochen.
In: Nature Methods, Vol. 12, No. 3, 26.02.2015, p. 199-202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Real-time deformability cytometry
T2 - On-the-fly cell mechanical phenotyping
AU - Otto, Oliver
AU - Rosendahl, Philipp
AU - Mietke, Alexander
AU - Golfier, Stefan
AU - Herold, Christoph
AU - Klaue, Daniel
AU - Girardo, Salvatore
AU - Pagliara, Stefano
AU - Ekpenyong, Andrew
AU - Jacobi, Angela
AU - Wobus, Manja
AU - Töpfner, Nicole
AU - Keyser, Ulrich F.
AU - Mansfeld, Jörg
AU - Fischer-Friedrich, Elisabeth
AU - Guck, Jochen
PY - 2015/2/26
Y1 - 2015/2/26
N2 - We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
AB - We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
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UR - http://www.scopus.com/inward/citedby.url?scp=84923846469&partnerID=8YFLogxK
U2 - 10.1038/nmeth.3281
DO - 10.1038/nmeth.3281
M3 - Article
C2 - 25643151
AN - SCOPUS:84923846469
VL - 12
SP - 199
EP - 202
JO - Nature Methods
JF - Nature Methods
SN - 1548-7091
IS - 3
ER -