Real-time deformability cytometry: On-the-fly cell mechanical phenotyping

Oliver Otto, Philipp Rosendahl, Alexander Mietke, Stefan Golfier, Christoph Herold, Daniel Klaue, Salvatore Girardo, Stefano Pagliara, Andrew Ekpenyong, Angela Jacobi, Manja Wobus, Nicole Töpfner, Ulrich F. Keyser, Jörg Mansfeld, Elisabeth Fischer-Friedrich, Jochen Guck

Research output: Contribution to journalArticle

259 Scopus citations

Abstract

We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

Original languageEnglish (US)
Pages (from-to)199-202
Number of pages4
JournalNature Methods
Volume12
Issue number3
DOIs
StatePublished - Feb 26 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D., Girardo, S., Pagliara, S., Ekpenyong, A., Jacobi, A., Wobus, M., Töpfner, N., Keyser, U. F., Mansfeld, J., Fischer-Friedrich, E., & Guck, J. (2015). Real-time deformability cytometry: On-the-fly cell mechanical phenotyping. Nature Methods, 12(3), 199-202. https://doi.org/10.1038/nmeth.3281