Region-specific Expression of NMDA Receptor GluN2C Subunit in Parvalbumin-Positive Neurons and Astrocytes

Analysis of GluN2C Expression using a Novel Reporter Model

Aparna Ravikrishnan, Pauravi J. Gandhi, Gajanan P. Shelkar, Jinxu Liu, Ratnamala Pavuluri, Shashank M. Dravid

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hypofunction of NMDA receptors in parvalbumin (PV)-positive interneurons has been proposed as a potential mechanism for cortical abnormalities and symptoms in schizophrenia. GluN2C-containing receptors have been linked to this hypothesis due to the higher affinity of psychotomimetic doses of ketamine for GluN1/2C receptors. However, the precise cell-type expression of GluN2C subunit remains unknown. We describe the expression of the GluN2C subunit using a novel EGFP reporter model. We observed EGFP(GluN2C) localization in PV-positive neurons in the nucleus reticularis of the thalamus, globus pallidus externa and interna, ventral pallidum and substantia nigra. In contrast, EGFP(GluN2C)-expressing cells did not co-localize with PV-positive neurons in the cortex, striatum, hippocampus or amygdala. Instead, EGFP(GluN2C) expression in these regions co-localized with an astrocytic marker. We confirmed functional expression of GluN2C-containing receptors in the PV-neurons in substantia nigra and cortical astrocytes using electrophysiology. GluN2C was found to be enriched in several first-order and higher order thalamic nuclei. Interestingly, we found that a previous GluN2C β-gal reporter model excluded expression from PV-neurons and certain thalamic nuclei but exhibited expression in the retrosplenial cortex. GluN2C's unique distribution in neuronal and non-neuronal cells in a brain region-specific manner raises interesting questions regarding the role of GluN2C-containing receptors in the central nervous system.

Original languageEnglish (US)
Pages (from-to)49-62
Number of pages14
JournalNeuroscience
Volume380
DOIs
StatePublished - Jun 1 2018

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Parvalbumins
N-Methyl-D-Aspartate Receptors
Astrocytes
Neurons
Thalamic Nuclei
Substantia Nigra
Globus Pallidus
Electrophysiology
Ketamine
Interneurons
Amygdala
Thalamus
Hippocampus
Schizophrenia
Central Nervous System
Brain
NR2C NMDA receptor

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Region-specific Expression of NMDA Receptor GluN2C Subunit in Parvalbumin-Positive Neurons and Astrocytes : Analysis of GluN2C Expression using a Novel Reporter Model. / Ravikrishnan, Aparna; Gandhi, Pauravi J.; Shelkar, Gajanan P.; Liu, Jinxu; Pavuluri, Ratnamala; Dravid, Shashank M.

In: Neuroscience, Vol. 380, 01.06.2018, p. 49-62.

Research output: Contribution to journalArticle

Ravikrishnan, Aparna ; Gandhi, Pauravi J. ; Shelkar, Gajanan P. ; Liu, Jinxu ; Pavuluri, Ratnamala ; Dravid, Shashank M. / Region-specific Expression of NMDA Receptor GluN2C Subunit in Parvalbumin-Positive Neurons and Astrocytes : Analysis of GluN2C Expression using a Novel Reporter Model. In: Neuroscience. 2018 ; Vol. 380. pp. 49-62.
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AU - Gandhi, Pauravi J.

AU - Shelkar, Gajanan P.

AU - Liu, Jinxu

AU - Pavuluri, Ratnamala

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