Region-specific onset of handling-induced changes in corticotropin- releasing factor and glucocorticoid receptor expression

Kristina A. Simeone, Kristen L. Brunson, Sarit Avishai-Eliner, Yuncai Chen, Tallie Z. Baram

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Early-life experience including maternal care profoundly influences hormonal stress responses during adulthood. Daily handling on postnatal day (P) 2-9, eliciting augmented maternal care upon returning pups to their cage, permanently modifies the expression of the stress neuromodulators corticotropin-releasing factor (CRP) and glucocorticoid receptor (GR). We have previously demonstrated reduced liypothalamic CRF expression already at the end of the handling period, followed by enhanced hippocampal GB mRNA levels (by P45). However, the initial site(s) and time of onset of these enduring changes have remained unclear. Therefore, we used semiquantitative in situ hybridization to delineate the spatiotemporal evolution of CRF and GR expression throughout stress-regulatory brain regions in handled (compared with undisturbed) pups. Enhanced CRF mRNA expression was apparent in the amygdaloid central nucleus (ACe) of handled pups already by P6. By P9, the augmented CRF mRNA levels persisted in ACe, accompanied by increased peptide expression in the bed nucleus of the stria terminalis and reduced expression in the paraventricular nucleus. The earliest change in GR consisted of reduced expression in the ACe of handled pups on P9, a time point when hippocampal GR expression was not yet affected. Thus, altered gene expression in ACe, bed nucleus of the stria terminalis as well as paraventricular nucleus may contribute to the molecular cascade by which handling (and increased maternal care) influences the stress response long term.

Original languageEnglish
Pages (from-to)2702-2706
Number of pages5
JournalEndocrinology
Volume145
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Corticotropin-Releasing Hormone Receptors
Glucocorticoid Receptors
Septal Nuclei
Paraventricular Hypothalamic Nucleus
Mothers
Messenger RNA
Nucleic Acid Regulatory Sequences
In Situ Hybridization
Neurotransmitter Agents
Gene Expression
Peptides
Central Amygdaloid Nucleus
Brain

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Region-specific onset of handling-induced changes in corticotropin- releasing factor and glucocorticoid receptor expression. / Simeone, Kristina A.; Brunson, Kristen L.; Avishai-Eliner, Sarit; Chen, Yuncai; Baram, Tallie Z.

In: Endocrinology, Vol. 145, No. 6, 06.2004, p. 2702-2706.

Research output: Contribution to journalArticle

Simeone, Kristina A. ; Brunson, Kristen L. ; Avishai-Eliner, Sarit ; Chen, Yuncai ; Baram, Tallie Z. / Region-specific onset of handling-induced changes in corticotropin- releasing factor and glucocorticoid receptor expression. In: Endocrinology. 2004 ; Vol. 145, No. 6. pp. 2702-2706.
@article{346c116b718c46ad928f71262120c504,
title = "Region-specific onset of handling-induced changes in corticotropin- releasing factor and glucocorticoid receptor expression",
abstract = "Early-life experience including maternal care profoundly influences hormonal stress responses during adulthood. Daily handling on postnatal day (P) 2-9, eliciting augmented maternal care upon returning pups to their cage, permanently modifies the expression of the stress neuromodulators corticotropin-releasing factor (CRP) and glucocorticoid receptor (GR). We have previously demonstrated reduced liypothalamic CRF expression already at the end of the handling period, followed by enhanced hippocampal GB mRNA levels (by P45). However, the initial site(s) and time of onset of these enduring changes have remained unclear. Therefore, we used semiquantitative in situ hybridization to delineate the spatiotemporal evolution of CRF and GR expression throughout stress-regulatory brain regions in handled (compared with undisturbed) pups. Enhanced CRF mRNA expression was apparent in the amygdaloid central nucleus (ACe) of handled pups already by P6. By P9, the augmented CRF mRNA levels persisted in ACe, accompanied by increased peptide expression in the bed nucleus of the stria terminalis and reduced expression in the paraventricular nucleus. The earliest change in GR consisted of reduced expression in the ACe of handled pups on P9, a time point when hippocampal GR expression was not yet affected. Thus, altered gene expression in ACe, bed nucleus of the stria terminalis as well as paraventricular nucleus may contribute to the molecular cascade by which handling (and increased maternal care) influences the stress response long term.",
author = "Simeone, {Kristina A.} and Brunson, {Kristen L.} and Sarit Avishai-Eliner and Yuncai Chen and Baram, {Tallie Z.}",
year = "2004",
month = "6",
doi = "10.1210/en.2004-0111",
language = "English",
volume = "145",
pages = "2702--2706",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - Region-specific onset of handling-induced changes in corticotropin- releasing factor and glucocorticoid receptor expression

AU - Simeone, Kristina A.

AU - Brunson, Kristen L.

AU - Avishai-Eliner, Sarit

AU - Chen, Yuncai

AU - Baram, Tallie Z.

PY - 2004/6

Y1 - 2004/6

N2 - Early-life experience including maternal care profoundly influences hormonal stress responses during adulthood. Daily handling on postnatal day (P) 2-9, eliciting augmented maternal care upon returning pups to their cage, permanently modifies the expression of the stress neuromodulators corticotropin-releasing factor (CRP) and glucocorticoid receptor (GR). We have previously demonstrated reduced liypothalamic CRF expression already at the end of the handling period, followed by enhanced hippocampal GB mRNA levels (by P45). However, the initial site(s) and time of onset of these enduring changes have remained unclear. Therefore, we used semiquantitative in situ hybridization to delineate the spatiotemporal evolution of CRF and GR expression throughout stress-regulatory brain regions in handled (compared with undisturbed) pups. Enhanced CRF mRNA expression was apparent in the amygdaloid central nucleus (ACe) of handled pups already by P6. By P9, the augmented CRF mRNA levels persisted in ACe, accompanied by increased peptide expression in the bed nucleus of the stria terminalis and reduced expression in the paraventricular nucleus. The earliest change in GR consisted of reduced expression in the ACe of handled pups on P9, a time point when hippocampal GR expression was not yet affected. Thus, altered gene expression in ACe, bed nucleus of the stria terminalis as well as paraventricular nucleus may contribute to the molecular cascade by which handling (and increased maternal care) influences the stress response long term.

AB - Early-life experience including maternal care profoundly influences hormonal stress responses during adulthood. Daily handling on postnatal day (P) 2-9, eliciting augmented maternal care upon returning pups to their cage, permanently modifies the expression of the stress neuromodulators corticotropin-releasing factor (CRP) and glucocorticoid receptor (GR). We have previously demonstrated reduced liypothalamic CRF expression already at the end of the handling period, followed by enhanced hippocampal GB mRNA levels (by P45). However, the initial site(s) and time of onset of these enduring changes have remained unclear. Therefore, we used semiquantitative in situ hybridization to delineate the spatiotemporal evolution of CRF and GR expression throughout stress-regulatory brain regions in handled (compared with undisturbed) pups. Enhanced CRF mRNA expression was apparent in the amygdaloid central nucleus (ACe) of handled pups already by P6. By P9, the augmented CRF mRNA levels persisted in ACe, accompanied by increased peptide expression in the bed nucleus of the stria terminalis and reduced expression in the paraventricular nucleus. The earliest change in GR consisted of reduced expression in the ACe of handled pups on P9, a time point when hippocampal GR expression was not yet affected. Thus, altered gene expression in ACe, bed nucleus of the stria terminalis as well as paraventricular nucleus may contribute to the molecular cascade by which handling (and increased maternal care) influences the stress response long term.

UR - http://www.scopus.com/inward/record.url?scp=2542496943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542496943&partnerID=8YFLogxK

U2 - 10.1210/en.2004-0111

DO - 10.1210/en.2004-0111

M3 - Article

VL - 145

SP - 2702

EP - 2706

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -