Regulation of β-adrenoceptor density and function in rat vas deferens

John Marvin May, Peter W. Abel, Kenneth P. Minneman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

β-Adrenoceptor density and responsiveness were examined in rat vas deferens following surgical and pharmacological treatments. Receptor density was measured by Scatchard analysis of saturation isotherms of specific [125I]pindolol ([125I]PIN) binding in membrane homogenates. Functional responsiveness was measured by isoprenaline-induced inhibition of field stimulated (60 V, 1 ms, 0.1 Hz) or 40 mM K+-induced contractions. Four days following surgical denervation of vas deferens there was no change in the density of [125I]PIN binding sites, suggesting that these sites are not located on prejunctional neurons. Neither 7 day bilateral adrenalectomy, 21 day denervation, nor 7 days treatment with 10 mg/kg per day desmethylimipramine caused changes in either the potency of isoprenaline in inhibiting contraction or the density of [125I]PIN binding sites compared to controls. Infusion of 3 mg/kg per day isoprenaline for 8 days significantly reduced the potency of isoprenaline in inhibiting field stimulated contractions, reduced the maximum degree of inhibition, and reduced the density of [125I]PIN binding sites. These results suggest that β-adrenoceptor density and responsiveness in rat vas deferens are not affected by removal of adrenal hormones or neuronal stimulation, but that receptor density and responsiveness can be decreased by increasing the concentration of β-adrenoceptor agonists at the receptor. Therefore, β-adrenoceptors in rat vas deferens probably receive little tonic stimulation under normal circumstances.

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalEuropean Journal of Pharmacology
Volume122
Issue number2
DOIs
StatePublished - Mar 18 1986
Externally publishedYes

Fingerprint

Vas Deferens
Isoproterenol
Adrenergic Receptors
Binding Sites
Denervation
Pindolol
Desipramine
Adrenalectomy
Hormones
Pharmacology
Neurons
Membranes

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Regulation of β-adrenoceptor density and function in rat vas deferens. / May, John Marvin; Abel, Peter W.; Minneman, Kenneth P.

In: European Journal of Pharmacology, Vol. 122, No. 2, 18.03.1986, p. 221-229.

Research output: Contribution to journalArticle

May, John Marvin ; Abel, Peter W. ; Minneman, Kenneth P. / Regulation of β-adrenoceptor density and function in rat vas deferens. In: European Journal of Pharmacology. 1986 ; Vol. 122, No. 2. pp. 221-229.
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