Regulation of [3H] d-aspartate release from mammalian isolated retinae by hydrogen sulfide

Catherine A. Opere, Emmanuel M. Monjok, Kaustubh H. Kulkarni, Ya Fatou Njie, Sunny E. Ohia

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Hydrogen sulfide (H2S), can produce pharmacological effects on neural and non-neural tissues from several mammalian species. The present study investigates the pharmacological action of H2S, (using sodium hydrosulfide, NaHS, and/or sodium sulfide, Na2S as donors) on amino acid neurotransmission (using [3H] d-aspartate as a marker for glutamate) from isolated, superfused bovine and porcine retinae. Isolated neural retinae were incubated in Krebs solution containing [3H] d-aspartate at 37°C. Release of [3H] d-aspartate was elicited by high potassium (K+ 50 mM) pulse. Both NaHS and Na2S donors caused an inhibition of K+-evoked [3H] d-aspartate release from isolated bovine retinae without affecting basal [3H] d-aspartate efflux yielding IC50 values of 0.006 and 6 μm, respectively. Furthermore, NaHS inhibited depolarization-evoked release of [3H] d-aspartate from isolated porcine retinae with an IC 50 value of 8 μM. The inhibitory action of NaHS on [3H] d-aspartate release from porcine retinae was blocked by propargyglycine, a selective inhibitor of cystathionine γ-lyase (CSE). Our results indicate that H2S donors can inhibit amino acid neurotransmission from both isolated bovine and porcine retinae, an effect that is dependent, at least in part, on intramural biosynthesis of H2S.

Original languageEnglish
Pages (from-to)1962-1968
Number of pages7
JournalNeurochemical Research
Volume34
Issue number11
DOIs
StatePublished - 2009

Fingerprint

Hydrogen Sulfide
Aspartic Acid
Retina
Swine
Synaptic Transmission
Cystathionine
Pharmacology
Amino Acids
Lyases
Biosynthesis
Depolarization
Inhibitory Concentration 50
Glutamic Acid
Potassium
sodium bisulfide
Tissue
sodium sulfide

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Biochemistry

Cite this

Regulation of [3H] d-aspartate release from mammalian isolated retinae by hydrogen sulfide. / Opere, Catherine A.; Monjok, Emmanuel M.; Kulkarni, Kaustubh H.; Njie, Ya Fatou; Ohia, Sunny E.

In: Neurochemical Research, Vol. 34, No. 11, 2009, p. 1962-1968.

Research output: Contribution to journalArticle

Opere, Catherine A. ; Monjok, Emmanuel M. ; Kulkarni, Kaustubh H. ; Njie, Ya Fatou ; Ohia, Sunny E. / Regulation of [3H] d-aspartate release from mammalian isolated retinae by hydrogen sulfide. In: Neurochemical Research. 2009 ; Vol. 34, No. 11. pp. 1962-1968.
@article{a337013cdb9b4af2992d9369b5e77523,
title = "Regulation of [3H] d-aspartate release from mammalian isolated retinae by hydrogen sulfide",
abstract = "Hydrogen sulfide (H2S), can produce pharmacological effects on neural and non-neural tissues from several mammalian species. The present study investigates the pharmacological action of H2S, (using sodium hydrosulfide, NaHS, and/or sodium sulfide, Na2S as donors) on amino acid neurotransmission (using [3H] d-aspartate as a marker for glutamate) from isolated, superfused bovine and porcine retinae. Isolated neural retinae were incubated in Krebs solution containing [3H] d-aspartate at 37°C. Release of [3H] d-aspartate was elicited by high potassium (K+ 50 mM) pulse. Both NaHS and Na2S donors caused an inhibition of K+-evoked [3H] d-aspartate release from isolated bovine retinae without affecting basal [3H] d-aspartate efflux yielding IC50 values of 0.006 and 6 μm, respectively. Furthermore, NaHS inhibited depolarization-evoked release of [3H] d-aspartate from isolated porcine retinae with an IC 50 value of 8 μM. The inhibitory action of NaHS on [3H] d-aspartate release from porcine retinae was blocked by propargyglycine, a selective inhibitor of cystathionine γ-lyase (CSE). Our results indicate that H2S donors can inhibit amino acid neurotransmission from both isolated bovine and porcine retinae, an effect that is dependent, at least in part, on intramural biosynthesis of H2S.",
author = "Opere, {Catherine A.} and Monjok, {Emmanuel M.} and Kulkarni, {Kaustubh H.} and Njie, {Ya Fatou} and Ohia, {Sunny E.}",
year = "2009",
doi = "10.1007/s11064-009-9984-x",
language = "English",
volume = "34",
pages = "1962--1968",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "11",

}

TY - JOUR

T1 - Regulation of [3H] d-aspartate release from mammalian isolated retinae by hydrogen sulfide

AU - Opere, Catherine A.

AU - Monjok, Emmanuel M.

AU - Kulkarni, Kaustubh H.

AU - Njie, Ya Fatou

AU - Ohia, Sunny E.

PY - 2009

Y1 - 2009

N2 - Hydrogen sulfide (H2S), can produce pharmacological effects on neural and non-neural tissues from several mammalian species. The present study investigates the pharmacological action of H2S, (using sodium hydrosulfide, NaHS, and/or sodium sulfide, Na2S as donors) on amino acid neurotransmission (using [3H] d-aspartate as a marker for glutamate) from isolated, superfused bovine and porcine retinae. Isolated neural retinae were incubated in Krebs solution containing [3H] d-aspartate at 37°C. Release of [3H] d-aspartate was elicited by high potassium (K+ 50 mM) pulse. Both NaHS and Na2S donors caused an inhibition of K+-evoked [3H] d-aspartate release from isolated bovine retinae without affecting basal [3H] d-aspartate efflux yielding IC50 values of 0.006 and 6 μm, respectively. Furthermore, NaHS inhibited depolarization-evoked release of [3H] d-aspartate from isolated porcine retinae with an IC 50 value of 8 μM. The inhibitory action of NaHS on [3H] d-aspartate release from porcine retinae was blocked by propargyglycine, a selective inhibitor of cystathionine γ-lyase (CSE). Our results indicate that H2S donors can inhibit amino acid neurotransmission from both isolated bovine and porcine retinae, an effect that is dependent, at least in part, on intramural biosynthesis of H2S.

AB - Hydrogen sulfide (H2S), can produce pharmacological effects on neural and non-neural tissues from several mammalian species. The present study investigates the pharmacological action of H2S, (using sodium hydrosulfide, NaHS, and/or sodium sulfide, Na2S as donors) on amino acid neurotransmission (using [3H] d-aspartate as a marker for glutamate) from isolated, superfused bovine and porcine retinae. Isolated neural retinae were incubated in Krebs solution containing [3H] d-aspartate at 37°C. Release of [3H] d-aspartate was elicited by high potassium (K+ 50 mM) pulse. Both NaHS and Na2S donors caused an inhibition of K+-evoked [3H] d-aspartate release from isolated bovine retinae without affecting basal [3H] d-aspartate efflux yielding IC50 values of 0.006 and 6 μm, respectively. Furthermore, NaHS inhibited depolarization-evoked release of [3H] d-aspartate from isolated porcine retinae with an IC 50 value of 8 μM. The inhibitory action of NaHS on [3H] d-aspartate release from porcine retinae was blocked by propargyglycine, a selective inhibitor of cystathionine γ-lyase (CSE). Our results indicate that H2S donors can inhibit amino acid neurotransmission from both isolated bovine and porcine retinae, an effect that is dependent, at least in part, on intramural biosynthesis of H2S.

UR - http://www.scopus.com/inward/record.url?scp=70349603066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349603066&partnerID=8YFLogxK

U2 - 10.1007/s11064-009-9984-x

DO - 10.1007/s11064-009-9984-x

M3 - Article

VL - 34

SP - 1962

EP - 1968

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 11

ER -