Regulation of inducible AmpC beta-lactamase expression among enterobacteriaceae

Nancy D. Hanson, C. C. Sanders

Research output: Contribution to journalReview article

132 Citations (Scopus)

Abstract

AmpC B-lactamases are active-site serine enzymes that are primarily cephalosporinases. In many gram negative organisms, including Enterobacter spp., Citrobacter freundii, Serratia marcescens, Morganella morganii and Pseudomonas aeruginosa, the expression of chromosomal ampC genes is low but inducible in response to β-lactams and other stimuli. The current working model for AmpC induction requires exposure of bacterial cells to β-lactam drugs or other stimuli and is linked to the cell wall recycling pathway. Induction of ampC appears to involve several gene products associated with this pathway. These gene products include AmpR, AmpD, and AmpG. In addition, anhydro forms of cell wall precursor muropeptides are believed to act as cofactors for AmpC induction. These cofactors bind to the DNA binding protein, AmpR, and define the role of AmpR as activator. Recent debate has ensued in the literature as to the identification of the precursor muropeptide involved in the activation process. Two candidate muropeptides include 1,6-anhydro-N-acetylmuramic acid L-Ala-D-Glu-meso-diaminopimelic acid (anhydro-MurNAc-tripeptide) and anhydro-MurNAc-L-Ala-D-Glu-meso- diaminopimelic acid-D-Ala-D-Ala (pentapeptide). The intent of this review is to address the general mechanism involved in AmpC induction. In doing so, the genes and gene products required for the process of AmpC induction are described. In addition, we review the data addressing cell wall recycling as it relates to AmpC induction.

Original languageEnglish
Pages (from-to)881-894
Number of pages14
JournalCurrent Pharmaceutical Design
Volume5
Issue number11
StatePublished - Nov 1999

Fingerprint

Enterobacteriaceae
Diaminopimelic Acid
Cell Wall
Lactams
Genes
Morganella morganii
Citrobacter freundii
Enterobacter
Serratia marcescens
DNA-Binding Proteins
Pseudomonas aeruginosa
Serine
Catalytic Domain
AmpC beta-lactamases
Enzymes
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Regulation of inducible AmpC beta-lactamase expression among enterobacteriaceae. / Hanson, Nancy D.; Sanders, C. C.

In: Current Pharmaceutical Design, Vol. 5, No. 11, 11.1999, p. 881-894.

Research output: Contribution to journalReview article

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