Regulation of interleukin-10 secretion by histamine in TH2 cells and splenocytes

Natalia Osna, Kathleen Elliott, Manzoor M. Khan

Research output: Contribution to journalArticle

Abstract

Interleukin-10 is a potent suppressive factor that down-regulates cellular immune response via inhibition of the production of TH1 cytokines. Histamine shifts the TH1/TH2 balance from TH1 to TH2 cytokines making the effects of histamine on IL-10 secretion an important factor in this switch. This study was designed to assess the role of histamine in the regulation of IL-10 production and the involvement of PKA and STAT factors in this process. TH2 cells (D10.G4.1) and AKR/j splenocytes were pretreated with histamine at a concentration range of 10-8-10-5 M for 1 h and then activated with PMA + ionomycin or anti-CD3 for 24 h. The supernatants were collected and tested for IL-10 content by ELISA. Histamine stimulated IL-10 production in TH2 cells in a dose-dependent manner that was reversed by both H1- and H2-receptor antagonists and by PKA inhibitors H8 and Rp-cAMPS. Tyrphostin also reversed the stimulation of IL-10 secretion by histamine, indicating that STAT factors were involved in this process. The up-regulation of IL-10 production by histamine in splenocytes was accompanied by inhibitory effects of histamine on IFNγ production. The pretreatment of splenocytes with histamine in the presence of anti-IL-10 abrogated histamine-mediated inhibition of IFNγ production suggesting that the effects of histamine on IFNγ secretion were regulated by IL-10 in multi-cell system.

Original languageEnglish (US)
Pages (from-to)85-96
Number of pages12
JournalInternational Immunopharmacology
Volume1
Issue number1
DOIs
StatePublished - Mar 15 2001

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Tyrphostins
Inbred AKR Mouse
Histamine Receptors
Bucladesine
Th2 Cells
Ionomycin
Enzyme Inhibitors
Tetradecanoylphorbol Acetate
Cyclic AMP-Dependent Protein Kinases
Interleukin-10
Histamine
Interferon-gamma
Spleen
Cell Line
Cytokines
Histamine H1 Receptors
Histamine H2 Receptors
Cellular Immunity
Up-Regulation
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Regulation of interleukin-10 secretion by histamine in TH2 cells and splenocytes. / Osna, Natalia; Elliott, Kathleen; Khan, Manzoor M.

In: International Immunopharmacology, Vol. 1, No. 1, 15.03.2001, p. 85-96.

Research output: Contribution to journalArticle

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