Abstract
Interleukin-10 is a potent suppressive factor that down-regulates cellular immune response via inhibition of the production of TH1 cytokines. Histamine shifts the TH1/TH2 balance from TH1 to TH2 cytokines making the effects of histamine on IL-10 secretion an important factor in this switch. This study was designed to assess the role of histamine in the regulation of IL-10 production and the involvement of PKA and STAT factors in this process. TH2 cells (D10.G4.1) and AKR/j splenocytes were pretreated with histamine at a concentration range of 10-8-10-5 M for 1 h and then activated with PMA + ionomycin or anti-CD3 for 24 h. The supernatants were collected and tested for IL-10 content by ELISA. Histamine stimulated IL-10 production in TH2 cells in a dose-dependent manner that was reversed by both H1- and H2-receptor antagonists and by PKA inhibitors H8 and Rp-cAMPS. Tyrphostin also reversed the stimulation of IL-10 secretion by histamine, indicating that STAT factors were involved in this process. The up-regulation of IL-10 production by histamine in splenocytes was accompanied by inhibitory effects of histamine on IFNγ production. The pretreatment of splenocytes with histamine in the presence of anti-IL-10 abrogated histamine-mediated inhibition of IFNγ production suggesting that the effects of histamine on IFNγ secretion were regulated by IL-10 in multi-cell system.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-96 |
| Number of pages | 12 |
| Journal | International Immunopharmacology |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 15 2001 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
- Pharmacology
Cite this
Regulation of interleukin-10 secretion by histamine in TH2 cells and splenocytes. / Osna, Natalia; Elliott, Kathleen; Khan, Manzoor M.
In: International Immunopharmacology, Vol. 1, No. 1, 15.03.2001, p. 85-96.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of interleukin-10 secretion by histamine in TH2 cells and splenocytes
AU - Osna, Natalia
AU - Elliott, Kathleen
AU - Khan, Manzoor M.
PY - 2001/3/15
Y1 - 2001/3/15
N2 - Interleukin-10 is a potent suppressive factor that down-regulates cellular immune response via inhibition of the production of TH1 cytokines. Histamine shifts the TH1/TH2 balance from TH1 to TH2 cytokines making the effects of histamine on IL-10 secretion an important factor in this switch. This study was designed to assess the role of histamine in the regulation of IL-10 production and the involvement of PKA and STAT factors in this process. TH2 cells (D10.G4.1) and AKR/j splenocytes were pretreated with histamine at a concentration range of 10-8-10-5 M for 1 h and then activated with PMA + ionomycin or anti-CD3 for 24 h. The supernatants were collected and tested for IL-10 content by ELISA. Histamine stimulated IL-10 production in TH2 cells in a dose-dependent manner that was reversed by both H1- and H2-receptor antagonists and by PKA inhibitors H8 and Rp-cAMPS. Tyrphostin also reversed the stimulation of IL-10 secretion by histamine, indicating that STAT factors were involved in this process. The up-regulation of IL-10 production by histamine in splenocytes was accompanied by inhibitory effects of histamine on IFNγ production. The pretreatment of splenocytes with histamine in the presence of anti-IL-10 abrogated histamine-mediated inhibition of IFNγ production suggesting that the effects of histamine on IFNγ secretion were regulated by IL-10 in multi-cell system.
AB - Interleukin-10 is a potent suppressive factor that down-regulates cellular immune response via inhibition of the production of TH1 cytokines. Histamine shifts the TH1/TH2 balance from TH1 to TH2 cytokines making the effects of histamine on IL-10 secretion an important factor in this switch. This study was designed to assess the role of histamine in the regulation of IL-10 production and the involvement of PKA and STAT factors in this process. TH2 cells (D10.G4.1) and AKR/j splenocytes were pretreated with histamine at a concentration range of 10-8-10-5 M for 1 h and then activated with PMA + ionomycin or anti-CD3 for 24 h. The supernatants were collected and tested for IL-10 content by ELISA. Histamine stimulated IL-10 production in TH2 cells in a dose-dependent manner that was reversed by both H1- and H2-receptor antagonists and by PKA inhibitors H8 and Rp-cAMPS. Tyrphostin also reversed the stimulation of IL-10 secretion by histamine, indicating that STAT factors were involved in this process. The up-regulation of IL-10 production by histamine in splenocytes was accompanied by inhibitory effects of histamine on IFNγ production. The pretreatment of splenocytes with histamine in the presence of anti-IL-10 abrogated histamine-mediated inhibition of IFNγ production suggesting that the effects of histamine on IFNγ secretion were regulated by IL-10 in multi-cell system.
UR - http://www.scopus.com/inward/record.url?scp=0035124146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035124146&partnerID=8YFLogxK
U2 - 10.1016/S0162-3109(00)00268-X
DO - 10.1016/S0162-3109(00)00268-X
M3 - Article
C2 - 11367520
AN - SCOPUS:0035124146
VL - 1
SP - 85
EP - 96
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 1
ER -