Regulation of norepinephrine release from isolated bovine irides by histamine

Kaustubh H. Kulkarni, Catherine A. Opere, Angela M. LeDay, Mohd A. Shara, Sunny E. Ohia

Research output: Contribution to journalArticle

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Abstract

In the present study, we investigated the effect of histamine on sympathetic neurotransmission from isolated, superfused bovine irides. We also studied the pharmacology of prejunctional histamine receptors that regulate the release of norepinephrine (NE) from this tissue. The effect of exogenous histamine and various histamine receptor agonists was examined on the release of [3H]-norepinephrine ([3H]NE) triggered by electrical field stimulation using the Superfusion Method. Histamine receptor agonists caused a concentration-dependent inhibition of field-stimulated [ 3H]NE overflow with the following rank order of potency: imetit > histamine > R-α-methylhistamine. In all cases, the inhibitory action of histamine receptor agonists was attenuated at high concentrations of these compounds. The histamine receptor antagonists, clobenpropit (H 3-antagonist/H4-agonist) and thioperamide (H 3-antagonist) blocked the inhibitory response elicited by R-α-methylhistamine and imetit, respectively. Inhibitory effects of R-α-methylhistamine and clonidine were not additive suggesting that prejunctional H3- and α2-adrenoceptors coexist at neurotransmitter release sites. We conclude that histamine produces an inhibitory action on sympathetic neurotransmission in the bovine iris, an effect mimicked by selective H3-receptor agonists and blocked by H 3-antagonists.

Original languageEnglish
Pages (from-to)891-897
Number of pages7
JournalNeurochemical Research
Volume31
Issue number7
DOIs
StatePublished - Jul 2006

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Histamine Receptors
Histamine Agonists
Iris
Histamine
Methylhistamines
Norepinephrine
thioperamide
Synaptic Transmission
Histamine H3 Receptors
Histamine Antagonists
Clonidine
Adrenergic Receptors
Electric Stimulation
Neurotransmitter Agents
Pharmacology
Tissue

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry

Cite this

Regulation of norepinephrine release from isolated bovine irides by histamine. / Kulkarni, Kaustubh H.; Opere, Catherine A.; LeDay, Angela M.; Shara, Mohd A.; Ohia, Sunny E.

In: Neurochemical Research, Vol. 31, No. 7, 07.2006, p. 891-897.

Research output: Contribution to journalArticle

Kulkarni, Kaustubh H. ; Opere, Catherine A. ; LeDay, Angela M. ; Shara, Mohd A. ; Ohia, Sunny E. / Regulation of norepinephrine release from isolated bovine irides by histamine. In: Neurochemical Research. 2006 ; Vol. 31, No. 7. pp. 891-897.
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