Regulation of retinal morphology and posterior segment amino acids by 8-isoprostaglandin E2 in bovine eyes ex vivo

Min Zhao, Christopher J. Destache, Guilin Zhan, Hong Liu, Yan Zhang, Venkatesh Govindarajan, Catherine A. Opere

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

There is evidence that isoprostanes (IsoPs) can regulate exogenously applied excitatory amino acid neurotransmitters in bovine retina in vitro. However, the regulation of retinal morphology and endogenous neurotransmitter levels by IsoPs is unknown. We examined the effects of intravitreally injected 8-iso-PGE2 on retinal tissue integrity and viability and amino acid neurotransmitters in bovine eye organ culture ex vivo. Exposure of bovine eyeballs to simulated experimental conditions revealed no retinal apoptosis and necrosis in TUNEL and DAPI staining and hematoxylin and eosin staining assays, respectively, and no changes in basal levels of amino acids in retina and vitreous humor Furthermore, intravitreal injection of 8-iso-PGE2 into bovine eyeballs had no effect on retinal apoptosis and integrity. Interestingly, 8-iso-PGE2 caused a concentration-dependent attenuation of retinal glutamate and its metabolite glutamine and glycine levels, while GABA was unaffected. 8-Iso-PGE2 (1 and 100 μM) significantly (P <0.001) attenuated glutamate levels by 33.9% and 48.0%, respectively. 8-Iso-PGE2 (100 μM) inhibited (P 2 can regulate retinal amino acids without inducing cell death in bovine retina ex vivo.

Original languageEnglish (US)
Pages (from-to)615-626
Number of pages12
JournalMethods and Findings in Experimental and Clinical Pharmacology
Volume30
Issue number8
DOIs
StatePublished - Oct 2008

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Regulation of retinal morphology and posterior segment amino acids by 8-isoprostaglandin E<sub>2</sub> in bovine eyes ex vivo'. Together they form a unique fingerprint.

Cite this