Regulation of [ 3H]D-aspartate release by the 5-F 2t-isoprostane and its 5-epimer in isolated bovine retina

Jamal Jamil, Ashley Wright, Na'Cara Harrison, Edem Kegey, Arnecia Faye Flowers, Namonique Jarell Flyod, Casey Kotera, Alexandre Guy, Jean Marie Galano, Thierry Durand, Ya Fatou Njie-Mbye, Sunny E. Ohia, Catherine A. Opere

Research output: Contribution to journalArticle

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Abstract

We have evidence that 15-F 2-isoprostanes (15-F 2-IsoPs) regulate excitatory neurotransmitter release in ocular tissues. Although 5-F 2-IsoPs are abundantly produced in mammals, their pharmacological actions on neurotransmitter release remain unknown. In the present study, we compared the effect of the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP (C5-OH in β-position) and 5-epi-5-F 2t-IsoP (C5-OH in α-position), on K +-evoked [ 3H]D-aspartate release in isolated bovine retina. We further examined the role of prostanoid receptors on the inhibitory action of 5-epi-5-F 2t-IsoP on [ 3H]D-aspartate overflow. Isolated bovine retina were prepared for studies of K +-evoked release of [ 3H]D-aspartate using the superfusion method. 5-epi-5-F 2t-IsoP (0.01 nM to 1 μM), attenuated K +-evoked [ 3H]D-aspartate release in a concentration-dependent manner, with the inhibitory effect of 26.9% (P <0.001; IC 25 = 0.2 μM) being achieved at 1 μM concentration. Its 5-(S)-OH-epimer, 5-F 2t-IsoP (0.1 nM-1 μM), exhibited an inhibitory biphasic action, yielding a maximal response of 35.7% (P <0.001) at 10 nM concentration of the drug (IC 25 value of 3 nM). Although the prostanoid-receptor antagonists, AH 6809 (10 μM; EP 1-3/DP) and BAY-u3405 (10 μM; DP/Tx) exhibited no effect on 5-epi-5-F 2t-IsoP (10 nM-1 μM)-mediated inhibition, SC-19220 (1 μM; EP 1) completely reversed 5-epi-5-F 2t-IsoP (0.1 μM and 1 μM)- induced attenuation of K +-evoked [ 3H]D-aspartate release. Similarly, both SC-51322 (10 μM; EP 1) and AH 23848 (1 μM; EP 4) reversed the inhibitory action elicited by 5-epi-5-F 2t-IsoP (0.1 μM) on the neurotransmitter release. We conclude that the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP and 5-epi-5-F 2t-IsoP, attenuate K +-induced [ 3H]D-aspartate release in isolated bovine retina presumably via prostanoid receptor dependent mechanisms. The trans-orientation of the allylic hydroxyl group at position C5 accounts for the apparent biphasic response exhibited by 5-F 2t-IsoP on excitatory neurotransmitter release.

Original languageEnglish
Pages (from-to)574-582
Number of pages9
JournalNeurochemical Research
Volume37
Issue number3
DOIs
StatePublished - Mar 2012

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D-Aspartic Acid
Retina
Neurotransmitter Agents
Prostaglandins
Dibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid, 8-chloro-, 2-acetylhydrazide
5-F(2t)-isoprostane
Isoprostanes
Mammals
Hydroxyl Radical
Tissue

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Biochemistry

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Regulation of [ 3H]D-aspartate release by the 5-F 2t-isoprostane and its 5-epimer in isolated bovine retina. / Jamil, Jamal; Wright, Ashley; Harrison, Na'Cara; Kegey, Edem; Flowers, Arnecia Faye; Flyod, Namonique Jarell; Kotera, Casey; Guy, Alexandre; Galano, Jean Marie; Durand, Thierry; Njie-Mbye, Ya Fatou; Ohia, Sunny E.; Opere, Catherine A.

In: Neurochemical Research, Vol. 37, No. 3, 03.2012, p. 574-582.

Research output: Contribution to journalArticle

Jamil, J, Wright, A, Harrison, NC, Kegey, E, Flowers, AF, Flyod, NJ, Kotera, C, Guy, A, Galano, JM, Durand, T, Njie-Mbye, YF, Ohia, SE & Opere, CA 2012, 'Regulation of [ 3H]D-aspartate release by the 5-F 2t-isoprostane and its 5-epimer in isolated bovine retina', Neurochemical Research, vol. 37, no. 3, pp. 574-582. https://doi.org/10.1007/s11064-011-0645-5
Jamil, Jamal ; Wright, Ashley ; Harrison, Na'Cara ; Kegey, Edem ; Flowers, Arnecia Faye ; Flyod, Namonique Jarell ; Kotera, Casey ; Guy, Alexandre ; Galano, Jean Marie ; Durand, Thierry ; Njie-Mbye, Ya Fatou ; Ohia, Sunny E. ; Opere, Catherine A. / Regulation of [ 3H]D-aspartate release by the 5-F 2t-isoprostane and its 5-epimer in isolated bovine retina. In: Neurochemical Research. 2012 ; Vol. 37, No. 3. pp. 574-582.
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abstract = "We have evidence that 15-F 2-isoprostanes (15-F 2-IsoPs) regulate excitatory neurotransmitter release in ocular tissues. Although 5-F 2-IsoPs are abundantly produced in mammals, their pharmacological actions on neurotransmitter release remain unknown. In the present study, we compared the effect of the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP (C5-OH in β-position) and 5-epi-5-F 2t-IsoP (C5-OH in α-position), on K +-evoked [ 3H]D-aspartate release in isolated bovine retina. We further examined the role of prostanoid receptors on the inhibitory action of 5-epi-5-F 2t-IsoP on [ 3H]D-aspartate overflow. Isolated bovine retina were prepared for studies of K +-evoked release of [ 3H]D-aspartate using the superfusion method. 5-epi-5-F 2t-IsoP (0.01 nM to 1 μM), attenuated K +-evoked [ 3H]D-aspartate release in a concentration-dependent manner, with the inhibitory effect of 26.9{\%} (P <0.001; IC 25 = 0.2 μM) being achieved at 1 μM concentration. Its 5-(S)-OH-epimer, 5-F 2t-IsoP (0.1 nM-1 μM), exhibited an inhibitory biphasic action, yielding a maximal response of 35.7{\%} (P <0.001) at 10 nM concentration of the drug (IC 25 value of 3 nM). Although the prostanoid-receptor antagonists, AH 6809 (10 μM; EP 1-3/DP) and BAY-u3405 (10 μM; DP/Tx) exhibited no effect on 5-epi-5-F 2t-IsoP (10 nM-1 μM)-mediated inhibition, SC-19220 (1 μM; EP 1) completely reversed 5-epi-5-F 2t-IsoP (0.1 μM and 1 μM)- induced attenuation of K +-evoked [ 3H]D-aspartate release. Similarly, both SC-51322 (10 μM; EP 1) and AH 23848 (1 μM; EP 4) reversed the inhibitory action elicited by 5-epi-5-F 2t-IsoP (0.1 μM) on the neurotransmitter release. We conclude that the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP and 5-epi-5-F 2t-IsoP, attenuate K +-induced [ 3H]D-aspartate release in isolated bovine retina presumably via prostanoid receptor dependent mechanisms. The trans-orientation of the allylic hydroxyl group at position C5 accounts for the apparent biphasic response exhibited by 5-F 2t-IsoP on excitatory neurotransmitter release.",
author = "Jamal Jamil and Ashley Wright and Na'Cara Harrison and Edem Kegey and Flowers, {Arnecia Faye} and Flyod, {Namonique Jarell} and Casey Kotera and Alexandre Guy and Galano, {Jean Marie} and Thierry Durand and Njie-Mbye, {Ya Fatou} and Ohia, {Sunny E.} and Opere, {Catherine A.}",
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T1 - Regulation of [ 3H]D-aspartate release by the 5-F 2t-isoprostane and its 5-epimer in isolated bovine retina

AU - Jamil, Jamal

AU - Wright, Ashley

AU - Harrison, Na'Cara

AU - Kegey, Edem

AU - Flowers, Arnecia Faye

AU - Flyod, Namonique Jarell

AU - Kotera, Casey

AU - Guy, Alexandre

AU - Galano, Jean Marie

AU - Durand, Thierry

AU - Njie-Mbye, Ya Fatou

AU - Ohia, Sunny E.

AU - Opere, Catherine A.

PY - 2012/3

Y1 - 2012/3

N2 - We have evidence that 15-F 2-isoprostanes (15-F 2-IsoPs) regulate excitatory neurotransmitter release in ocular tissues. Although 5-F 2-IsoPs are abundantly produced in mammals, their pharmacological actions on neurotransmitter release remain unknown. In the present study, we compared the effect of the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP (C5-OH in β-position) and 5-epi-5-F 2t-IsoP (C5-OH in α-position), on K +-evoked [ 3H]D-aspartate release in isolated bovine retina. We further examined the role of prostanoid receptors on the inhibitory action of 5-epi-5-F 2t-IsoP on [ 3H]D-aspartate overflow. Isolated bovine retina were prepared for studies of K +-evoked release of [ 3H]D-aspartate using the superfusion method. 5-epi-5-F 2t-IsoP (0.01 nM to 1 μM), attenuated K +-evoked [ 3H]D-aspartate release in a concentration-dependent manner, with the inhibitory effect of 26.9% (P <0.001; IC 25 = 0.2 μM) being achieved at 1 μM concentration. Its 5-(S)-OH-epimer, 5-F 2t-IsoP (0.1 nM-1 μM), exhibited an inhibitory biphasic action, yielding a maximal response of 35.7% (P <0.001) at 10 nM concentration of the drug (IC 25 value of 3 nM). Although the prostanoid-receptor antagonists, AH 6809 (10 μM; EP 1-3/DP) and BAY-u3405 (10 μM; DP/Tx) exhibited no effect on 5-epi-5-F 2t-IsoP (10 nM-1 μM)-mediated inhibition, SC-19220 (1 μM; EP 1) completely reversed 5-epi-5-F 2t-IsoP (0.1 μM and 1 μM)- induced attenuation of K +-evoked [ 3H]D-aspartate release. Similarly, both SC-51322 (10 μM; EP 1) and AH 23848 (1 μM; EP 4) reversed the inhibitory action elicited by 5-epi-5-F 2t-IsoP (0.1 μM) on the neurotransmitter release. We conclude that the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP and 5-epi-5-F 2t-IsoP, attenuate K +-induced [ 3H]D-aspartate release in isolated bovine retina presumably via prostanoid receptor dependent mechanisms. The trans-orientation of the allylic hydroxyl group at position C5 accounts for the apparent biphasic response exhibited by 5-F 2t-IsoP on excitatory neurotransmitter release.

AB - We have evidence that 15-F 2-isoprostanes (15-F 2-IsoPs) regulate excitatory neurotransmitter release in ocular tissues. Although 5-F 2-IsoPs are abundantly produced in mammals, their pharmacological actions on neurotransmitter release remain unknown. In the present study, we compared the effect of the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP (C5-OH in β-position) and 5-epi-5-F 2t-IsoP (C5-OH in α-position), on K +-evoked [ 3H]D-aspartate release in isolated bovine retina. We further examined the role of prostanoid receptors on the inhibitory action of 5-epi-5-F 2t-IsoP on [ 3H]D-aspartate overflow. Isolated bovine retina were prepared for studies of K +-evoked release of [ 3H]D-aspartate using the superfusion method. 5-epi-5-F 2t-IsoP (0.01 nM to 1 μM), attenuated K +-evoked [ 3H]D-aspartate release in a concentration-dependent manner, with the inhibitory effect of 26.9% (P <0.001; IC 25 = 0.2 μM) being achieved at 1 μM concentration. Its 5-(S)-OH-epimer, 5-F 2t-IsoP (0.1 nM-1 μM), exhibited an inhibitory biphasic action, yielding a maximal response of 35.7% (P <0.001) at 10 nM concentration of the drug (IC 25 value of 3 nM). Although the prostanoid-receptor antagonists, AH 6809 (10 μM; EP 1-3/DP) and BAY-u3405 (10 μM; DP/Tx) exhibited no effect on 5-epi-5-F 2t-IsoP (10 nM-1 μM)-mediated inhibition, SC-19220 (1 μM; EP 1) completely reversed 5-epi-5-F 2t-IsoP (0.1 μM and 1 μM)- induced attenuation of K +-evoked [ 3H]D-aspartate release. Similarly, both SC-51322 (10 μM; EP 1) and AH 23848 (1 μM; EP 4) reversed the inhibitory action elicited by 5-epi-5-F 2t-IsoP (0.1 μM) on the neurotransmitter release. We conclude that the 5-F 2-IsoP epimer pair, 5-F 2t-IsoP and 5-epi-5-F 2t-IsoP, attenuate K +-induced [ 3H]D-aspartate release in isolated bovine retina presumably via prostanoid receptor dependent mechanisms. The trans-orientation of the allylic hydroxyl group at position C5 accounts for the apparent biphasic response exhibited by 5-F 2t-IsoP on excitatory neurotransmitter release.

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