Release of Luminal Exosomes Contributes to TLR4-Mediated Epithelial Antimicrobial Defense

Guoku Hu, Ai Yu Gong, Amanda L. Roth, Bing Q. Huang, Honorine D. Ward, Guan Zhu, Nicholas F. LaRusso, Nancy D. Hanson, Xian-Ming Chen

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Exosomes are membranous nanovesicles released by most cell types from multi-vesicular endosomes. They are speculated to transfer molecules to neighboring or distant cells and modulate many physiological and pathological procedures. Exosomes released from the gastrointestinal epithelium to the basolateral side have been implicated in antigen presentation. Here, we report that luminal release of exosomes from the biliary and intestinal epithelium is increased following infection by the protozoan parasite Cryptosporidium parvum. Release of exosomes involves activation of TLR4/IKK2 signaling through promoting the SNAP23-associated vesicular exocytotic process. Downregulation of let-7 family miRNAs by activation of TLR4 signaling increases SNAP23 expression, coordinating exosome release in response to C. parvum infection. Intriguingly, exosomes carry antimicrobial peptides of epithelial cell origin, including cathelicidin-37 and beta-defensin 2. Activation of TLR4 signaling enhances exosomal shuttle of epithelial antimicrobial peptides. Exposure of C. parvum sporozoites to released exosomes decreases their viability and infectivity both in vitro and ex vivo. Direct binding to the C. parvum sporozoite surface is required for the anti-C. parvum activity of released exosomes. Biliary epithelial cells also increase exosomal release and display exosome-associated anti-C. parvum activity following LPS stimulation. Our data indicate that TLR4 signaling regulates luminal exosome release and shuttling of antimicrobial peptides from the gastrointestinal epithelium, revealing a new arm of mucosal immunity relevant to antimicrobial defense.

Original languageEnglish
Article numbere1003261
JournalPLoS Pathogens
Volume9
Issue number4
DOIs
StatePublished - Apr 2013

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Exosomes
Cryptosporidium parvum
Sporozoites
Peptides
Epithelium
Epithelial Cells
Protozoan Infections
beta-Defensins
Mucosal Immunity
Parasitic Diseases
Endosomes
Antigen Presentation
Intestinal Mucosa
MicroRNAs
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Release of Luminal Exosomes Contributes to TLR4-Mediated Epithelial Antimicrobial Defense. / Hu, Guoku; Gong, Ai Yu; Roth, Amanda L.; Huang, Bing Q.; Ward, Honorine D.; Zhu, Guan; LaRusso, Nicholas F.; Hanson, Nancy D.; Chen, Xian-Ming.

In: PLoS Pathogens, Vol. 9, No. 4, e1003261, 04.2013.

Research output: Contribution to journalArticle

Hu, Guoku ; Gong, Ai Yu ; Roth, Amanda L. ; Huang, Bing Q. ; Ward, Honorine D. ; Zhu, Guan ; LaRusso, Nicholas F. ; Hanson, Nancy D. ; Chen, Xian-Ming. / Release of Luminal Exosomes Contributes to TLR4-Mediated Epithelial Antimicrobial Defense. In: PLoS Pathogens. 2013 ; Vol. 9, No. 4.
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