Replication efficiency of soil-bound prions varies with soil type

Samuel E. Saunders, Ronald A. Shikiya, Katie Langenfeld, Shannon L. Bartelt-Hunt, Jason C. Bartz

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Prion sorption to soil is thought to play an important role in the transmission of scrapie and chronic wasting disease (CWD) via the environment. Sorption of PrP to soil and soil minerals is influenced by the strain and species of PrP Sc and by soil characteristics. However, the ability of soil-bound prions to convert PrP c to PrP Sc under these wide-ranging conditions remains poorly understood. We developed a semiquantitative protein misfolding cyclic amplification (PMCA) protocol to evaluate replication efficiency of soil-bound prions. Binding of the hyper (HY) strain of transmissible mink encephalopathy (TME) (hamster) prions to a silty clay loam soil yielded a greater-than-1-log decrease in PMCA replication efficiency with a corresponding 1.3-log reduction in titer. The increased binding of PrP Sc to soil over time corresponded with a decrease in PMCA replication efficiency. The PMCA efficiency of bound prions varied with soil type, where prions bound to clay and organic surfaces exhibited significantly lower replication efficiencies while prions bound to sand exhibited no apparent difference in replication efficiency compared to unbound controls. PMCA results from hamster and CWD agent-infected elk prions yielded similar findings. Given that PrP Sc adsorption affinity varies with soil type, the overall balance between prion adsorption affinity and replication efficiency for the dominant soil types of an area may be a significant determinant in the environmental transmission of prion diseases.

Original languageEnglish
Pages (from-to)5476-5482
Number of pages7
JournalJournal of Virology
Volume85
Issue number11
DOIs
StatePublished - Jun 2011

Fingerprint

Prions
prions
soil types
Soil
soil
chronic wasting disease
Chronic Wasting Disease
hamsters
proteins
sorption
Proteins
transmissible mink encephalopathy
adsorption
Cricetinae
Adsorption
prion diseases
scrapie
elks
Mink
Scrapie

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Saunders, S. E., Shikiya, R. A., Langenfeld, K., Bartelt-Hunt, S. L., & Bartz, J. C. (2011). Replication efficiency of soil-bound prions varies with soil type. Journal of Virology, 85(11), 5476-5482. https://doi.org/10.1128/JVI.00282-11

Replication efficiency of soil-bound prions varies with soil type. / Saunders, Samuel E.; Shikiya, Ronald A.; Langenfeld, Katie; Bartelt-Hunt, Shannon L.; Bartz, Jason C.

In: Journal of Virology, Vol. 85, No. 11, 06.2011, p. 5476-5482.

Research output: Contribution to journalArticle

Saunders, SE, Shikiya, RA, Langenfeld, K, Bartelt-Hunt, SL & Bartz, JC 2011, 'Replication efficiency of soil-bound prions varies with soil type', Journal of Virology, vol. 85, no. 11, pp. 5476-5482. https://doi.org/10.1128/JVI.00282-11
Saunders SE, Shikiya RA, Langenfeld K, Bartelt-Hunt SL, Bartz JC. Replication efficiency of soil-bound prions varies with soil type. Journal of Virology. 2011 Jun;85(11):5476-5482. https://doi.org/10.1128/JVI.00282-11
Saunders, Samuel E. ; Shikiya, Ronald A. ; Langenfeld, Katie ; Bartelt-Hunt, Shannon L. ; Bartz, Jason C. / Replication efficiency of soil-bound prions varies with soil type. In: Journal of Virology. 2011 ; Vol. 85, No. 11. pp. 5476-5482.
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