Requirement for the βI and βIV tubulin isotypes in mammalian cilia

Heather C. Jensen-Smith, Richard F. Ludueña, Richard J. Hallworth

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

[Nielsen et al., 2001: Curr Biol 11:529-533], based on studies in Drosophila, have proposed that β tubulin in axonemal microtubules must contain a specific acidic seven amino acid sequence in its carboxyl terminus. In mammals, the two βIV isotypes (βIVa and βIVb) contain that sequence. In order to test the application of this hypothesis to mammals, we have examined the expression of β tubulin isotypes in four different ciliated tissues (trachea, ependyma, uterine tube, and testis) using isotype-specific antibodies and indirect immunofluorescence. We find that βIV tubulin is present in all ciliated cell types examined, but so is β1 tubulin. Taken together with recent studies that show that β1 and βIV tubulin are both present in the cilia of vestibular hair cells, olfactory neurons, and nasal respiratory epithelial cells, we propose that both β1 tubulin and βIV tubulin may be required for axonemal structures in mammals.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalCell Motility and the Cytoskeleton
Volume55
Issue number3
DOIs
StatePublished - Jul 1 2003

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Cilia
Tubulin
Mammals
Vestibular Hair Cells
Ependyma
Acidic Amino Acids
Fallopian Tubes
Indirect Fluorescent Antibody Technique
Trachea
Nose
Microtubules
Drosophila
Testis
Amino Acid Sequence
Epithelial Cells
Neurons
Antibodies

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Requirement for the βI and βIV tubulin isotypes in mammalian cilia. / Jensen-Smith, Heather C.; Ludueña, Richard F.; Hallworth, Richard J.

In: Cell Motility and the Cytoskeleton, Vol. 55, No. 3, 01.07.2003, p. 213-220.

Research output: Contribution to journalArticle

Jensen-Smith, Heather C. ; Ludueña, Richard F. ; Hallworth, Richard J. / Requirement for the βI and βIV tubulin isotypes in mammalian cilia. In: Cell Motility and the Cytoskeleton. 2003 ; Vol. 55, No. 3. pp. 213-220.
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