Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope

Chad E. Bigelow, Curtis J. Harkrider, David L. Conover, Thomas H. Foster, Irene Georgakoudi, Soumya Mitra, Michael G. Nichols, Milind Rajadhyaksha

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We describe the design and implementation of an inverted laser scanning confocal fluorescence microscope utilizing the commercial Nikon Diaphot TMD platform. An external confocal scanner was retrofitted through the video side port of the Diaphot. With 10×, 0.5 NA dry and 60×, 1.4 NA oil immersion objectives, the depth discrimination is 5.8 μm and 0.8 μm, respectively, as determined by derivatives of fluorescence edge responses measured in liquid samples of rhodamine 6G dissolved in DMSO. We present sample edge response curves and representative confocal fluorescence images of tumor cells in monolayer culture.

Original languageEnglish
Pages (from-to)3407-3410
Number of pages4
JournalReview of Scientific Instruments
Volume72
Issue number8
DOIs
StatePublished - Aug 2001

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scanners
Microscopes
Fluorescence
microscopes
fluorescence
Lasers
lasers
rhodamine
Cell culture
submerging
discrimination
Tumors
Monolayers
tumors
platforms
oils
Cells
Derivatives
Scanning
scanning

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)
  • Instrumentation

Cite this

Bigelow, C. E., Harkrider, C. J., Conover, D. L., Foster, T. H., Georgakoudi, I., Mitra, S., ... Rajadhyaksha, M. (2001). Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope. Review of Scientific Instruments, 72(8), 3407-3410. https://doi.org/10.1063/1.1382637

Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope. / Bigelow, Chad E.; Harkrider, Curtis J.; Conover, David L.; Foster, Thomas H.; Georgakoudi, Irene; Mitra, Soumya; Nichols, Michael G.; Rajadhyaksha, Milind.

In: Review of Scientific Instruments, Vol. 72, No. 8, 08.2001, p. 3407-3410.

Research output: Contribution to journalArticle

Bigelow, CE, Harkrider, CJ, Conover, DL, Foster, TH, Georgakoudi, I, Mitra, S, Nichols, MG & Rajadhyaksha, M 2001, 'Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope', Review of Scientific Instruments, vol. 72, no. 8, pp. 3407-3410. https://doi.org/10.1063/1.1382637
Bigelow CE, Harkrider CJ, Conover DL, Foster TH, Georgakoudi I, Mitra S et al. Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope. Review of Scientific Instruments. 2001 Aug;72(8):3407-3410. https://doi.org/10.1063/1.1382637
Bigelow, Chad E. ; Harkrider, Curtis J. ; Conover, David L. ; Foster, Thomas H. ; Georgakoudi, Irene ; Mitra, Soumya ; Nichols, Michael G. ; Rajadhyaksha, Milind. / Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope. In: Review of Scientific Instruments. 2001 ; Vol. 72, No. 8. pp. 3407-3410.
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