Reversibility of scrapie inactivation is enhanced by copper

Debbie McKenzie, Jason C. Bartz, Jean Mirwald, Doris Olander, Richard Marsh, Judd Aiken

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

The only known difference between the cellular (PrP(C)) and scrapie- specific (PrP(Sc)) isoforms of the prion protein is conformational. Because disruption of PrP(Sc) structure decreases scrapie infectivity, restoration of the disease-specific conformation should restore infectivity. In this study, disruption of PrP(Sc) (as monitored by the loss of proteinase K resistance) by guanidine hydrochloride (GdnHCl) resulted in decreased infectivity. Upon dilution of the GdnHCl, protease resistance of PrP was restored and infectivity was regained. The addition of copper facilitated restoration of both infectivity and protease resistance of PrP in a subset of samples that did not renature by the simple dilution of the GdnHCl. These data demonstrate that loss of scrapie infectivity can be a reversible process and that copper can enhance this restoration of proteinase K resistance and infectivity.

Original languageEnglish
Pages (from-to)25545-25547
Number of pages3
JournalJournal of Biological Chemistry
Volume273
Issue number40
DOIs
StatePublished - Oct 2 1998
Externally publishedYes

Fingerprint

Scrapie
Guanidine
Restoration
Copper
Endopeptidase K
Dilution
Peptide Hydrolases
PrPSc Proteins
Conformations
Protein Isoforms

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

McKenzie, D., Bartz, J. C., Mirwald, J., Olander, D., Marsh, R., & Aiken, J. (1998). Reversibility of scrapie inactivation is enhanced by copper. Journal of Biological Chemistry, 273(40), 25545-25547. https://doi.org/10.1074/jbc.273.40.25545

Reversibility of scrapie inactivation is enhanced by copper. / McKenzie, Debbie; Bartz, Jason C.; Mirwald, Jean; Olander, Doris; Marsh, Richard; Aiken, Judd.

In: Journal of Biological Chemistry, Vol. 273, No. 40, 02.10.1998, p. 25545-25547.

Research output: Contribution to journalArticle

McKenzie, D, Bartz, JC, Mirwald, J, Olander, D, Marsh, R & Aiken, J 1998, 'Reversibility of scrapie inactivation is enhanced by copper', Journal of Biological Chemistry, vol. 273, no. 40, pp. 25545-25547. https://doi.org/10.1074/jbc.273.40.25545
McKenzie, Debbie ; Bartz, Jason C. ; Mirwald, Jean ; Olander, Doris ; Marsh, Richard ; Aiken, Judd. / Reversibility of scrapie inactivation is enhanced by copper. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 40. pp. 25545-25547.
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