Role of catalase in pre- and postjunctional responses of mammalian irides to hydrogen peroxide

B. Matutte, S. O. Awe, F. A. Ameh, A. M. Leday, J. C. Rice, Catherine A. Opere, S. E. Ohia

Research output: Contribution to journalArticle

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Abstract

In the present study, we examined the effect of inhibition of catalase with 3-amino-triazole (3-AT) on hydrogen peroxide (H2O2)-induced enhancement of sympathetic neuro-transmission in bovine irides and on the inhibitory effect of this oxidant on norepinephrine (NE) release from human irides, in vitro. Furthermore, we investigated the effect of 3-AT on H2O2-induced attenuation of contractile responses to carbachol in the bovine isolated irides. Isolated mammalian irides were prepared for studies of [3H]NE release using the superfusion method and for contractile studies using isolated organ baths. At concentrations less than 100 μM, H2O2 had no significant effect on field-stimulated [3H]NE release from bovine or human irides. In bovine irides, 3-AT caused significant (P <0.001) leftward shifts of concentration-response curves to H2O2 (10 - 300 μM). 3-AT also increased H2O2-induced attenuation of evoked [3H]NE release from human isolated irides. Low concentrations of H2O2 (<100 μM) had no effect on carbachol contractions. However, 3-AT unmasked an inhibitory effect of low concentrations of H2O2 (3 - 100 μM) on carbachol-induced contractions. We conclude that inhibition of catalase causes both pre- and postjunctional responses of isolated mammalian irides to be more susceptible to oxidative stress induced by H2O2.

Original languageEnglish
Pages (from-to)429-438
Number of pages10
JournalJournal of Ocular Pharmacology and Therapeutics
Volume16
Issue number5
StatePublished - 2000

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Iris
Catalase
Hydrogen Peroxide
Triazoles
Norepinephrine
Carbachol
Baths
Oxidants
Oxidative Stress

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Matutte, B., Awe, S. O., Ameh, F. A., Leday, A. M., Rice, J. C., Opere, C. A., & Ohia, S. E. (2000). Role of catalase in pre- and postjunctional responses of mammalian irides to hydrogen peroxide. Journal of Ocular Pharmacology and Therapeutics, 16(5), 429-438.

Role of catalase in pre- and postjunctional responses of mammalian irides to hydrogen peroxide. / Matutte, B.; Awe, S. O.; Ameh, F. A.; Leday, A. M.; Rice, J. C.; Opere, Catherine A.; Ohia, S. E.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 16, No. 5, 2000, p. 429-438.

Research output: Contribution to journalArticle

Matutte, B, Awe, SO, Ameh, FA, Leday, AM, Rice, JC, Opere, CA & Ohia, SE 2000, 'Role of catalase in pre- and postjunctional responses of mammalian irides to hydrogen peroxide', Journal of Ocular Pharmacology and Therapeutics, vol. 16, no. 5, pp. 429-438.
Matutte, B. ; Awe, S. O. ; Ameh, F. A. ; Leday, A. M. ; Rice, J. C. ; Opere, Catherine A. ; Ohia, S. E. / Role of catalase in pre- and postjunctional responses of mammalian irides to hydrogen peroxide. In: Journal of Ocular Pharmacology and Therapeutics. 2000 ; Vol. 16, No. 5. pp. 429-438.
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