TY - JOUR
T1 - Role of intracellular calcium in peroxide-induced potentiation of norepinephrine release from bovine isolated irides
AU - Opere, Catherine A.
AU - Ohia, Sunny E.
N1 - Funding Information:
The authors thank both the Greater Omaha Packing Co. and the J. F. O’Neill Packing Co., Omaha, Nebraska, for their generous donations of cow eyeballs. We are grateful to Ms. Dawn Trojanowski for her excellent secretarial assistance. This study was supported by the National Institutes of Health (Grant #EY11023).
PY - 1998/11
Y1 - 1998/11
N2 - 1. BAPTA AM (10 μM), thapsigargin (10 μM), ruthenium red (30 μM) and oligomycin (30 μM) inhibited field-stimulated [3H]NE release from bovine isolated irides by 54%, 30%, 30% and 26%, respectively. 2. Both BAPTA AM and thapsigargin had no significant effect (P>0.05) on H2O2-induced potentiation of evoked [3H]NE release. 3. Ruthenium red prevented (but oligomycin enhanced) H2O2-induced enhancement of evoked [3H]NE release. 4. We conclude that, although intracellular calcium participates in field-stimulation evoked [3H]NE release from bovine isolated irides, only the mitochondrial pool of calcium may be involved in peroxide-induced enhancement of sympathetic neurotransmission. Copyright (C) 1998 Elsevier Science Inc.
AB - 1. BAPTA AM (10 μM), thapsigargin (10 μM), ruthenium red (30 μM) and oligomycin (30 μM) inhibited field-stimulated [3H]NE release from bovine isolated irides by 54%, 30%, 30% and 26%, respectively. 2. Both BAPTA AM and thapsigargin had no significant effect (P>0.05) on H2O2-induced potentiation of evoked [3H]NE release. 3. Ruthenium red prevented (but oligomycin enhanced) H2O2-induced enhancement of evoked [3H]NE release. 4. We conclude that, although intracellular calcium participates in field-stimulation evoked [3H]NE release from bovine isolated irides, only the mitochondrial pool of calcium may be involved in peroxide-induced enhancement of sympathetic neurotransmission. Copyright (C) 1998 Elsevier Science Inc.
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U2 - 10.1016/S0306-3623(98)00090-1
DO - 10.1016/S0306-3623(98)00090-1
M3 - Article
C2 - 9809480
AN - SCOPUS:0032213225
VL - 31
SP - 793
EP - 798
JO - Vascular Pharmacology
JF - Vascular Pharmacology
SN - 1537-1891
IS - 5
ER -