Role of intracellular calcium in peroxide-induced potentiation of norepinephrine release from bovine isolated irides

Catherine A. Opere, Sunny E. Ohia

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. BAPTA AM (10 μM), thapsigargin (10 μM), ruthenium red (30 μM) and oligomycin (30 μM) inhibited field-stimulated [3H]NE release from bovine isolated irides by 54%, 30%, 30% and 26%, respectively. 2. Both BAPTA AM and thapsigargin had no significant effect (P>0.05) on H2O2-induced potentiation of evoked [3H]NE release. 3. Ruthenium red prevented (but oligomycin enhanced) H2O2-induced enhancement of evoked [3H]NE release. 4. We conclude that, although intracellular calcium participates in field-stimulation evoked [3H]NE release from bovine isolated irides, only the mitochondrial pool of calcium may be involved in peroxide-induced enhancement of sympathetic neurotransmission. Copyright (C) 1998 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)793-798
Number of pages6
JournalGeneral Pharmacology
Volume31
Issue number5
DOIs
StatePublished - Nov 1998

Fingerprint

Oligomycins
Ruthenium Red
Thapsigargin
Iris
Norepinephrine
Calcium
Peroxides
Synaptic Transmission
calcium peroxide
1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Role of intracellular calcium in peroxide-induced potentiation of norepinephrine release from bovine isolated irides. / Opere, Catherine A.; Ohia, Sunny E.

In: General Pharmacology, Vol. 31, No. 5, 11.1998, p. 793-798.

Research output: Contribution to journalArticle

@article{e502a45d3a564ea1a03381f06338f4af,
title = "Role of intracellular calcium in peroxide-induced potentiation of norepinephrine release from bovine isolated irides",
abstract = "1. BAPTA AM (10 μM), thapsigargin (10 μM), ruthenium red (30 μM) and oligomycin (30 μM) inhibited field-stimulated [3H]NE release from bovine isolated irides by 54{\%}, 30{\%}, 30{\%} and 26{\%}, respectively. 2. Both BAPTA AM and thapsigargin had no significant effect (P>0.05) on H2O2-induced potentiation of evoked [3H]NE release. 3. Ruthenium red prevented (but oligomycin enhanced) H2O2-induced enhancement of evoked [3H]NE release. 4. We conclude that, although intracellular calcium participates in field-stimulation evoked [3H]NE release from bovine isolated irides, only the mitochondrial pool of calcium may be involved in peroxide-induced enhancement of sympathetic neurotransmission. Copyright (C) 1998 Elsevier Science Inc.",
author = "Opere, {Catherine A.} and Ohia, {Sunny E.}",
year = "1998",
month = "11",
doi = "10.1016/S0306-3623(98)00090-1",
language = "English",
volume = "31",
pages = "793--798",
journal = "Vascular Pharmacology",
issn = "1537-1891",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Role of intracellular calcium in peroxide-induced potentiation of norepinephrine release from bovine isolated irides

AU - Opere, Catherine A.

AU - Ohia, Sunny E.

PY - 1998/11

Y1 - 1998/11

N2 - 1. BAPTA AM (10 μM), thapsigargin (10 μM), ruthenium red (30 μM) and oligomycin (30 μM) inhibited field-stimulated [3H]NE release from bovine isolated irides by 54%, 30%, 30% and 26%, respectively. 2. Both BAPTA AM and thapsigargin had no significant effect (P>0.05) on H2O2-induced potentiation of evoked [3H]NE release. 3. Ruthenium red prevented (but oligomycin enhanced) H2O2-induced enhancement of evoked [3H]NE release. 4. We conclude that, although intracellular calcium participates in field-stimulation evoked [3H]NE release from bovine isolated irides, only the mitochondrial pool of calcium may be involved in peroxide-induced enhancement of sympathetic neurotransmission. Copyright (C) 1998 Elsevier Science Inc.

AB - 1. BAPTA AM (10 μM), thapsigargin (10 μM), ruthenium red (30 μM) and oligomycin (30 μM) inhibited field-stimulated [3H]NE release from bovine isolated irides by 54%, 30%, 30% and 26%, respectively. 2. Both BAPTA AM and thapsigargin had no significant effect (P>0.05) on H2O2-induced potentiation of evoked [3H]NE release. 3. Ruthenium red prevented (but oligomycin enhanced) H2O2-induced enhancement of evoked [3H]NE release. 4. We conclude that, although intracellular calcium participates in field-stimulation evoked [3H]NE release from bovine isolated irides, only the mitochondrial pool of calcium may be involved in peroxide-induced enhancement of sympathetic neurotransmission. Copyright (C) 1998 Elsevier Science Inc.

UR - http://www.scopus.com/inward/record.url?scp=0032213225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032213225&partnerID=8YFLogxK

U2 - 10.1016/S0306-3623(98)00090-1

DO - 10.1016/S0306-3623(98)00090-1

M3 - Article

VL - 31

SP - 793

EP - 798

JO - Vascular Pharmacology

JF - Vascular Pharmacology

SN - 1537-1891

IS - 5

ER -