Selected contribution

Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress

G. Patrick Lambert, C. V. Gisolfi, D. J. Berg, P. L. Moseley, L. W. Oberley, K. C. Kregel

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

The purpose of this study was to characterize intestinal permeability changes over a range of physiologically relevant body temperatures in vivo and in vitro. Initially, FITC-dextran (4,000 Da), a large fluorescent molecule, was loaded into the small intestine of anesthetized rats. The rats were then maintained at ∼37°C or heated over 90 min to a core body temperature of ∼41, ∼41.5, or ∼42.5°C. Permeability was greater in the 42.5°C group compared with the 37, 41, or 41.5°C groups. Histological analysis revealed intestinal epithelial damage in heated groups. Everted intestinal sacs were then used to further characterize hyperthermia-induced intestinal permeability and to study the potential role of oxidative and nitrosative stress. Increased permeability to 4,000-Da FITC-dextran in both small intestinal and colonic sacs was observed at a temperature of 41.5-42°C compared with 37°C, along with widespread intestinal epithelial damage. Administration of antioxidant enzyme mimics or a nitric oxide synthase inhibitor did not reduce permeability due to heat stress, and tissue concentrations of a lipid peroxidation product were not altered by heat stress, suggesting that oxidative and nitrosative stress were not likely mediators of this phenomenon in vitro. In conclusion, hyperthermia produced increased permeability and marked intestinal epithelial damage both in vivo and in vitro, suggesting that thermal disruption of epithelial membranes contributes to the intestinal barrier dysfunction manifested with heat stress.

Original languageEnglish (US)
Pages (from-to)1750-1761
Number of pages12
JournalJournal of Applied Physiology
Volume92
Issue number4
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

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Induced Hyperthermia
Permeability
Oxidative Stress
Hot Temperature
Body Temperature
Nitric Oxide Synthase
Lipid Peroxidation
Small Intestine
Fever
Antioxidants
Temperature
Membranes
Enzymes
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Selected contribution : Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress. / Lambert, G. Patrick; Gisolfi, C. V.; Berg, D. J.; Moseley, P. L.; Oberley, L. W.; Kregel, K. C.

In: Journal of Applied Physiology, Vol. 92, No. 4, 01.01.2002, p. 1750-1761.

Research output: Contribution to journalArticle

Lambert, G. Patrick ; Gisolfi, C. V. ; Berg, D. J. ; Moseley, P. L. ; Oberley, L. W. ; Kregel, K. C. / Selected contribution : Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress. In: Journal of Applied Physiology. 2002 ; Vol. 92, No. 4. pp. 1750-1761.
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