Separation of blood cells with differing deformability using deterministic lateral displacement

David Holmes, Graeme Whyte, Joe Bailey, Nuria Vergara-Irigaray, Andrew Ekpenyong, Jochen Guck, Tom Duke

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Determining cell mechanical properties is increasingly recognized as a marker-free way to characterize and separate biological cells. This emerging realization has led to the development of a plethora of appropriate measurement techniques. Here, we use a fairly novel approach, deterministic lateral displacement (DLD), to separate blood cells based on their mechanical phenotype with high throughput. Human red blood cells were treated chemically to alter their membrane deformability and the effect of this alteration on the hydrodynamic behaviour of the cells in a DLD device was investigated. Cells of defined stiffness (glutaraldehyde cross-linked erythro-cytes) were used to test the performance of the DLD device across a range of cell stiffness and applied shear rates. Optical stretching was used as an independent method for quantifying the variation in stiffness of the cells. Lateral displacement of cells flowing within the device, and their subsequent exit position from the device were shown to correlate with cell stiffness. Data showing how the isolation of leucocytes from whole blood varies with applied shear rate are also presented. The ability to sort leucocyte sub-populations (T-lymphocytes and neutrophils), based on a combination of cell size and deformability, demonstrates the potential for using DLD devices to perform continuous fractionation and/or enrichment of leucocyte sub-populations from whole blood.

Original languageEnglish
Article number20140011
JournalInterface Focus
Volume4
Issue number6
DOIs
StatePublished - Dec 6 2014
Externally publishedYes

Fingerprint

Formability
Blood Cells
Blood
Cells
Stiffness
Shear deformation
Equipment and Supplies
Leukocytes
T-cells
Glutaral
Fractionation
Stretching
Hydrodynamics
Throughput
Membranes
Mechanical properties
Cell Size
Population
Neutrophils
Erythrocytes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biotechnology
  • Biochemistry
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials

Cite this

Separation of blood cells with differing deformability using deterministic lateral displacement. / Holmes, David; Whyte, Graeme; Bailey, Joe; Vergara-Irigaray, Nuria; Ekpenyong, Andrew; Guck, Jochen; Duke, Tom.

In: Interface Focus, Vol. 4, No. 6, 20140011, 06.12.2014.

Research output: Contribution to journalArticle

Holmes, D, Whyte, G, Bailey, J, Vergara-Irigaray, N, Ekpenyong, A, Guck, J & Duke, T 2014, 'Separation of blood cells with differing deformability using deterministic lateral displacement', Interface Focus, vol. 4, no. 6, 20140011. https://doi.org/10.1098/rsfs.2014.0011
Holmes, David ; Whyte, Graeme ; Bailey, Joe ; Vergara-Irigaray, Nuria ; Ekpenyong, Andrew ; Guck, Jochen ; Duke, Tom. / Separation of blood cells with differing deformability using deterministic lateral displacement. In: Interface Focus. 2014 ; Vol. 4, No. 6.
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