Abstract
We have recently identified two unrelated kindreds in which DR1 and DR2 co-segregate as a single haplotype spanning several generations. Serology, PCR-RFLP and sequence-specific oligonucleotide probes were used to characterize the presence and segregation of the DRB1 and DRB5 loci in these two kindreds. In both families, the recombination resulted in co-expression of a DRB1 locus that encoded a DR1 serologic phenotype and a DRB5 locus that encoded a 'short' DR2 serologic phenotype. One of these kindreds co-expressed the DR1 and DR15 (DRB5(*)01). The other kindred co-expressed the DR1 and DR16 (DRB5(*)02). Our data indicate that the recombination event occurred in the region between the DRB1 locus and the DRB5 locus. This recombinant haplotype produces a DR2 phenotype which lacks the epitopes normally encoded by the DRB1 locus, resulting in a serologic 'short' antigen. The clinical significance of such a recombinational event becomes evident when patients with such a genotype require allogeneic bone marrow transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 148-154 |
| Number of pages | 7 |
| Journal | Tissue Antigens |
| Volume | 41 |
| Issue number | 3 |
| State | Published - 1993 |
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All Science Journal Classification (ASJC) codes
- Cell Biology
- Immunology
Cite this
Serologic and molecular studies of two kindreds expressing recombinant HLA DR1/DR2 haplotypes. / Wisecarver, J.; Shepherd, S.; Beisel, Kirk; Rubocki, A. R.
In: Tissue Antigens, Vol. 41, No. 3, 1993, p. 148-154.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Serologic and molecular studies of two kindreds expressing recombinant HLA DR1/DR2 haplotypes
AU - Wisecarver, J.
AU - Shepherd, S.
AU - Beisel, Kirk
AU - Rubocki, A. R.
PY - 1993
Y1 - 1993
N2 - We have recently identified two unrelated kindreds in which DR1 and DR2 co-segregate as a single haplotype spanning several generations. Serology, PCR-RFLP and sequence-specific oligonucleotide probes were used to characterize the presence and segregation of the DRB1 and DRB5 loci in these two kindreds. In both families, the recombination resulted in co-expression of a DRB1 locus that encoded a DR1 serologic phenotype and a DRB5 locus that encoded a 'short' DR2 serologic phenotype. One of these kindreds co-expressed the DR1 and DR15 (DRB5(*)01). The other kindred co-expressed the DR1 and DR16 (DRB5(*)02). Our data indicate that the recombination event occurred in the region between the DRB1 locus and the DRB5 locus. This recombinant haplotype produces a DR2 phenotype which lacks the epitopes normally encoded by the DRB1 locus, resulting in a serologic 'short' antigen. The clinical significance of such a recombinational event becomes evident when patients with such a genotype require allogeneic bone marrow transplantation.
AB - We have recently identified two unrelated kindreds in which DR1 and DR2 co-segregate as a single haplotype spanning several generations. Serology, PCR-RFLP and sequence-specific oligonucleotide probes were used to characterize the presence and segregation of the DRB1 and DRB5 loci in these two kindreds. In both families, the recombination resulted in co-expression of a DRB1 locus that encoded a DR1 serologic phenotype and a DRB5 locus that encoded a 'short' DR2 serologic phenotype. One of these kindreds co-expressed the DR1 and DR15 (DRB5(*)01). The other kindred co-expressed the DR1 and DR16 (DRB5(*)02). Our data indicate that the recombination event occurred in the region between the DRB1 locus and the DRB5 locus. This recombinant haplotype produces a DR2 phenotype which lacks the epitopes normally encoded by the DRB1 locus, resulting in a serologic 'short' antigen. The clinical significance of such a recombinational event becomes evident when patients with such a genotype require allogeneic bone marrow transplantation.
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M3 - Article
VL - 41
SP - 148
EP - 154
JO - HLA
JF - HLA
SN - 2059-2302
IS - 3
ER -