Serologic and molecular studies of two kindreds expressing recombinant HLA DR1/DR2 haplotypes

J. Wisecarver, S. Shepherd, Kirk Beisel, A. R. Rubocki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have recently identified two unrelated kindreds in which DR1 and DR2 co-segregate as a single haplotype spanning several generations. Serology, PCR-RFLP and sequence-specific oligonucleotide probes were used to characterize the presence and segregation of the DRB1 and DRB5 loci in these two kindreds. In both families, the recombination resulted in co-expression of a DRB1 locus that encoded a DR1 serologic phenotype and a DRB5 locus that encoded a 'short' DR2 serologic phenotype. One of these kindreds co-expressed the DR1 and DR15 (DRB5(*)01). The other kindred co-expressed the DR1 and DR16 (DRB5(*)02). Our data indicate that the recombination event occurred in the region between the DRB1 locus and the DRB5 locus. This recombinant haplotype produces a DR2 phenotype which lacks the epitopes normally encoded by the DRB1 locus, resulting in a serologic 'short' antigen. The clinical significance of such a recombinational event becomes evident when patients with such a genotype require allogeneic bone marrow transplantation.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalTissue Antigens
Volume41
Issue number3
StatePublished - 1993

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HLA-DR1 Antigen
HLA-DR2 Antigen
Haplotypes
Phenotype
Genetic Recombination
Oligonucleotide Probes
Homologous Transplantation
Serology
Bone Marrow Transplantation
Restriction Fragment Length Polymorphisms
Epitopes
Genotype
Antigens
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Immunology

Cite this

Serologic and molecular studies of two kindreds expressing recombinant HLA DR1/DR2 haplotypes. / Wisecarver, J.; Shepherd, S.; Beisel, Kirk; Rubocki, A. R.

In: Tissue Antigens, Vol. 41, No. 3, 1993, p. 148-154.

Research output: Contribution to journalArticle

Wisecarver, J, Shepherd, S, Beisel, K & Rubocki, AR 1993, 'Serologic and molecular studies of two kindreds expressing recombinant HLA DR1/DR2 haplotypes', Tissue Antigens, vol. 41, no. 3, pp. 148-154.
Wisecarver, J. ; Shepherd, S. ; Beisel, Kirk ; Rubocki, A. R. / Serologic and molecular studies of two kindreds expressing recombinant HLA DR1/DR2 haplotypes. In: Tissue Antigens. 1993 ; Vol. 41, No. 3. pp. 148-154.
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