Sex hormone regulation of survivin gene expression

Nancy H. Nabilsi, Russell R. Broaddus, Adrienne S. McCampbell, Karen H. Lu, Henry T. Lynch, Lee May Chen, David S. Loose

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Survivin (BIRC5) is a cell survival gene that is overexpressed in endometrial cancer and has been implicated to have a physiological role in normal endometrial function. To determine whether survivin gene expression is regulated by reproductive steroid hormones in the human endometrium, RNA was prepared from normal cycling women in the proliferative and secretory phases of the menstrual cycle. RNA was also isolated from 21 endometrial biopsies from premenopausal women at baseline and following 3 months of treatment with depot medroxyprogesterone acetate. Finally, RNA was isolated from endometrial biopsies from ten healthy postmenopausal women participating in a clinical trial of estrogen replacement therapy at baseline and following 6 months of treatment with conjugated equine estrogen. Quantitative RT-PCR analysis was used to determine survivin, insulin-like growth factor binding protein 1 (IGFBP1), Ki67, and IGF1 gene expression levels. Survivin gene expression was highest in the proliferative phase of the menstrual cycle and showed a statistically significant 4-fold increase in expression following chronic treatment with estrogens; this was strongly correlated with increased Ki67, a marker of proliferation. Survivin gene expression decreased 4.6-fold following chronic progestin treatment in the human endometrium. These data suggest that survivin transcript is regulated by estrogens and progestins in the disease-free human endometrium. The data also suggest that survivin transcript may be used as a biomarker of estrogen and progestin treatment efficacy, but validation studies must be conducted to support this conclusion.

Original languageEnglish
Pages (from-to)237-243
Number of pages7
JournalJournal of Endocrinology
Volume207
Issue number2
DOIs
StatePublished - Nov 2010

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Gene Expression Regulation
Gonadal Steroid Hormones
Progestins
Endometrium
Gene Expression
Estrogens
Follicular Phase
RNA
Insulin-Like Growth Factor Binding Protein 1
Biopsy
Conjugated (USP) Estrogens
Medroxyprogesterone Acetate
Estrogen Replacement Therapy
Validation Studies
Luteal Phase
Therapeutics
Endometrial Neoplasms
Cell Survival
Biomarkers
Steroids

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Nabilsi, N. H., Broaddus, R. R., McCampbell, A. S., Lu, K. H., Lynch, H. T., Chen, L. M., & Loose, D. S. (2010). Sex hormone regulation of survivin gene expression. Journal of Endocrinology, 207(2), 237-243. https://doi.org/10.1677/JOE-10-0128

Sex hormone regulation of survivin gene expression. / Nabilsi, Nancy H.; Broaddus, Russell R.; McCampbell, Adrienne S.; Lu, Karen H.; Lynch, Henry T.; Chen, Lee May; Loose, David S.

In: Journal of Endocrinology, Vol. 207, No. 2, 11.2010, p. 237-243.

Research output: Contribution to journalArticle

Nabilsi, NH, Broaddus, RR, McCampbell, AS, Lu, KH, Lynch, HT, Chen, LM & Loose, DS 2010, 'Sex hormone regulation of survivin gene expression', Journal of Endocrinology, vol. 207, no. 2, pp. 237-243. https://doi.org/10.1677/JOE-10-0128
Nabilsi NH, Broaddus RR, McCampbell AS, Lu KH, Lynch HT, Chen LM et al. Sex hormone regulation of survivin gene expression. Journal of Endocrinology. 2010 Nov;207(2):237-243. https://doi.org/10.1677/JOE-10-0128
Nabilsi, Nancy H. ; Broaddus, Russell R. ; McCampbell, Adrienne S. ; Lu, Karen H. ; Lynch, Henry T. ; Chen, Lee May ; Loose, David S. / Sex hormone regulation of survivin gene expression. In: Journal of Endocrinology. 2010 ; Vol. 207, No. 2. pp. 237-243.
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