Short-wave diathermy pretreatment and inflammatory myokine response after high-intensity eccentric exercise

John P. Vardiman, Nicole Moodie, Jacob A. Siedlik, Rebecca A. Kudrna, Zachary Graham, Philip Gallagher

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Context: Various modalities have been used to pretreat skeletal muscle to attenuate inflammation. Objective: To determine the effects of short-wave diathermy (SWD) preheating treatment on inflammation and stress markers after eccentric exercise. Design: Controlled laboratory study. Setting: University laboratory setting. Patients or Other Participants: Fifteen male (age = 22 ± 4.9 years, height = 179.75 ± 9.56 cm, mass = 82.22 ± 12.67 kg) college-aged students. Intervention(s): Seven participants were selected randomly to receive 40 minutes of SWD heat treatment (HT), and 8 participants served as the control (CON) group and rested without SWD. Both groups completed 7 sets of 10 repetitions of a high-intensity eccentric exercise protocol (EEP) at 120% of the 1-repetition maximum (1-RM) leg extension. Main Outcome Measure(s): We biopsied muscles on days 1, 3 (24 hours post-EEP), and 4 (48 hours post-EEP) and collected blood samples on days 1, 2 (4 hours post-EEP), 3, and 4. We determined 1-RM on day 2 (24 hours post-SWD) and measured 1-RM on days 3 and 4. We analyzed the muscle samples for interleukin 6 (IL-6), tumor necrosis factor α, and heat shock protein 70 and the blood for serum creatine kinase. Results: We found a group × time interaction for intramuscular IL-6 levels after SWD (F2,26 = 7.13, P = .003). The IL- 6 decreased in HT (F1,6 = 17.8, P = .006), whereas CON showed no change (P > .05). We found a group × time interaction for tumor necrosis factor a levels (F2,26 = 3.71, P =.04), which increased in CON (F2,14 = 7.16, P = .007), but saw no changes for HT (P > .05). No group × time interactions were noted for 1-RM, heat shock protein 70, or creatine kinase (P > .05). Conclusions: The SWD preheating treatment provided a treatment effect for intramuscular inflammatory myokines induced through high-intensity eccentric exercise but did not affect other factors associated with intense exercise and inflammation.

Original languageEnglish (US)
Pages (from-to)612-620
Number of pages9
JournalJournal of Athletic Training
Volume50
Issue number6
DOIs
StatePublished - Jan 1 2015

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Radio Waves
Diathermy
Exercise
Interleukin-6
HSP70 Heat-Shock Proteins
Hot Temperature
Creatine Kinase
Inflammation
Therapeutics
Tumor Necrosis Factor-alpha
Muscles
Protein Kinases
Leg
Skeletal Muscle
Outcome Assessment (Health Care)
Students
Control Groups
Serum

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Short-wave diathermy pretreatment and inflammatory myokine response after high-intensity eccentric exercise. / Vardiman, John P.; Moodie, Nicole; Siedlik, Jacob A.; Kudrna, Rebecca A.; Graham, Zachary; Gallagher, Philip.

In: Journal of Athletic Training, Vol. 50, No. 6, 01.01.2015, p. 612-620.

Research output: Contribution to journalArticle

Vardiman, John P. ; Moodie, Nicole ; Siedlik, Jacob A. ; Kudrna, Rebecca A. ; Graham, Zachary ; Gallagher, Philip. / Short-wave diathermy pretreatment and inflammatory myokine response after high-intensity eccentric exercise. In: Journal of Athletic Training. 2015 ; Vol. 50, No. 6. pp. 612-620.
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abstract = "Context: Various modalities have been used to pretreat skeletal muscle to attenuate inflammation. Objective: To determine the effects of short-wave diathermy (SWD) preheating treatment on inflammation and stress markers after eccentric exercise. Design: Controlled laboratory study. Setting: University laboratory setting. Patients or Other Participants: Fifteen male (age = 22 ± 4.9 years, height = 179.75 ± 9.56 cm, mass = 82.22 ± 12.67 kg) college-aged students. Intervention(s): Seven participants were selected randomly to receive 40 minutes of SWD heat treatment (HT), and 8 participants served as the control (CON) group and rested without SWD. Both groups completed 7 sets of 10 repetitions of a high-intensity eccentric exercise protocol (EEP) at 120{\%} of the 1-repetition maximum (1-RM) leg extension. Main Outcome Measure(s): We biopsied muscles on days 1, 3 (24 hours post-EEP), and 4 (48 hours post-EEP) and collected blood samples on days 1, 2 (4 hours post-EEP), 3, and 4. We determined 1-RM on day 2 (24 hours post-SWD) and measured 1-RM on days 3 and 4. We analyzed the muscle samples for interleukin 6 (IL-6), tumor necrosis factor α, and heat shock protein 70 and the blood for serum creatine kinase. Results: We found a group × time interaction for intramuscular IL-6 levels after SWD (F2,26 = 7.13, P = .003). The IL- 6 decreased in HT (F1,6 = 17.8, P = .006), whereas CON showed no change (P > .05). We found a group × time interaction for tumor necrosis factor a levels (F2,26 = 3.71, P =.04), which increased in CON (F2,14 = 7.16, P = .007), but saw no changes for HT (P > .05). No group × time interactions were noted for 1-RM, heat shock protein 70, or creatine kinase (P > .05). Conclusions: The SWD preheating treatment provided a treatment effect for intramuscular inflammatory myokines induced through high-intensity eccentric exercise but did not affect other factors associated with intense exercise and inflammation.",
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AU - Graham, Zachary

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