Abstract
5-Aminosalicylic acid (5-ASA, mesalamine) is an antiinflammatory agent used in the treatment of Crohn's disease and ulcerative colitis. A simple LC method was developed and validated for the analysis of 5-ASA. The chromatographic separation was achieved on a reversed-phase, C8 column with UV detection at 290 nm. This isocratic system was operated at ambient temperature and required 8 min of chromatographic time. The mobile phase consisted of methanol;phosphate buffer pH 7.4 (20:80, v/v). The flow-rate was maintained at 1.0 ml/min. Standard curves were linear over the concentration range of 10 to 200 μg ml-1. Intra-day and inter-day relative standard deviations ranged from 1.1-3.5% and 1.2-4.6% respectively. This method was used to: (1) quantify 5-ASA in pharmaceutical formulations without any complex sample extraction procedure, and (2) study the stability of 5-ASA in Sorensen's phosphate buffer (pH 7.4), Sorensen's phosphate buffer containing 5 mg% (w/v) of Carbopol, and in pharmaceutical formulations.
| Original language | English |
|---|---|
| Pages (from-to) | 233-239 |
| Number of pages | 7 |
| Journal | Journal of Chromatography A |
| Volume | 765 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 28 1997 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Simple liquid chromatographic method for the analysis of 5-aminosalicylic acid and its degradation product. / Haney, P. W.; Dash, Alekha K.
In: Journal of Chromatography A, Vol. 765, No. 2, 28.03.1997, p. 233-239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simple liquid chromatographic method for the analysis of 5-aminosalicylic acid and its degradation product
AU - Haney, P. W.
AU - Dash, Alekha K.
PY - 1997/3/28
Y1 - 1997/3/28
N2 - 5-Aminosalicylic acid (5-ASA, mesalamine) is an antiinflammatory agent used in the treatment of Crohn's disease and ulcerative colitis. A simple LC method was developed and validated for the analysis of 5-ASA. The chromatographic separation was achieved on a reversed-phase, C8 column with UV detection at 290 nm. This isocratic system was operated at ambient temperature and required 8 min of chromatographic time. The mobile phase consisted of methanol;phosphate buffer pH 7.4 (20:80, v/v). The flow-rate was maintained at 1.0 ml/min. Standard curves were linear over the concentration range of 10 to 200 μg ml-1. Intra-day and inter-day relative standard deviations ranged from 1.1-3.5% and 1.2-4.6% respectively. This method was used to: (1) quantify 5-ASA in pharmaceutical formulations without any complex sample extraction procedure, and (2) study the stability of 5-ASA in Sorensen's phosphate buffer (pH 7.4), Sorensen's phosphate buffer containing 5 mg% (w/v) of Carbopol, and in pharmaceutical formulations.
AB - 5-Aminosalicylic acid (5-ASA, mesalamine) is an antiinflammatory agent used in the treatment of Crohn's disease and ulcerative colitis. A simple LC method was developed and validated for the analysis of 5-ASA. The chromatographic separation was achieved on a reversed-phase, C8 column with UV detection at 290 nm. This isocratic system was operated at ambient temperature and required 8 min of chromatographic time. The mobile phase consisted of methanol;phosphate buffer pH 7.4 (20:80, v/v). The flow-rate was maintained at 1.0 ml/min. Standard curves were linear over the concentration range of 10 to 200 μg ml-1. Intra-day and inter-day relative standard deviations ranged from 1.1-3.5% and 1.2-4.6% respectively. This method was used to: (1) quantify 5-ASA in pharmaceutical formulations without any complex sample extraction procedure, and (2) study the stability of 5-ASA in Sorensen's phosphate buffer (pH 7.4), Sorensen's phosphate buffer containing 5 mg% (w/v) of Carbopol, and in pharmaceutical formulations.
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U2 - 10.1016/S0021-9673(96)00921-1
DO - 10.1016/S0021-9673(96)00921-1
M3 - Article
VL - 765
SP - 233
EP - 239
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 2
ER -