Simultaneous estimation of nalidixic acid and metronidazole using uv/visible spectrophotometer

Gopal Jadhav, H. N. More, K. R. Mahadik

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Two accurate and economical procedures for simultaneous estimation of nalidixic acid and metronidazole in two component tablet formulations have been developed. The methods employ simultaneous equations and Q-analysis for the simultaneous estimation of nalidixic acid and metronidazole. In 50% acetonitrile 256 nm, absorbance maxima of nalidixic acid, 320 nm, absorbance maxima of metronidazole and 279 nm, isobestic point of both the drugs were used as a analytical wavelength. The Beer's Law range for nalidixic acid and metronidazole were found to be 0-22.5 μg/ml (regression equation is C = 9.544 ABS + 0.038) and 0-15 μg/ml (regression equation is C = 19.508 ABS + 0.094) respectively. The results of the analysis have been validated statistically and by recovery studies.

Original languageEnglish (US)
Pages (from-to)475-480
Number of pages6
JournalIndian Drugs
Volume35
Issue number8
StatePublished - Aug 1 1998
Externally publishedYes

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Nalidixic Acid
Metronidazole
Tablets
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Simultaneous estimation of nalidixic acid and metronidazole using uv/visible spectrophotometer. / Jadhav, Gopal; More, H. N.; Mahadik, K. R.

In: Indian Drugs, Vol. 35, No. 8, 01.08.1998, p. 475-480.

Research output: Contribution to journalArticle

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