Skeletal unloading and dietary copper depletion are detrimental to bone quality of mature rats

Brenda J. Smith, Jarrod B. King, Edralin A. Lucas, Mohammed P. Akhter, Bahram H. Arjmandi, Barbara J. Stoecker

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This study was designed to examine the skeletal response to copper depletion and mechanical unloading in mature animals. In a 2 × 2 experimental design, 5.5-mo-old male Sprague-Dawley rats (n = 36) consumed either the control (AIN-93M) or Cu-depletion (-Cu) diet beginning 21 d before suspension and throughout the remainder of the study. Half of the rats in each dietary treatment group were either tail-suspended (TS) or kept ambulatory (AMB) for 28 d. Lower bone mineral densities (BMD) of 5th lumbar vertebra (L5) (P <0.05) and femur were observed with -Cu and TS, but no differences were noted in the BMD of the humerus. Mechanical strength in the femur and vertebra decreased in response to TS, but were unaffected by copper depletion. Urinary deoxypyridinoline, an index of bone resorption, was significantly greater in TS rats, but unaltered by -Cu. No changes in serum or bone alkaline phosphatase activity, an indicator of bone formation, were observed. Our findings suggest that TS and -Cu decreased BMD in unloaded femur and vertebra but had no effect on normally loaded humerus. Bone loss with TS appeared to be related to accelerated bone resorption. Alterations in bone metabolism and bone mechanical properties in the mature skeleton resulting from -Cu warrant further investigation.

Original languageEnglish
Pages (from-to)190-196
Number of pages7
JournalJournal of Nutrition
Volume132
Issue number2
StatePublished - 2002

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Hindlimb Suspension
Tail
Copper
tail
copper
bones
Bone and Bones
rats
bone density
vertebrae
femur
Bone Density
Femur
bone resorption
humerus
Humerus
Bone Resorption
Spine
bone metabolism
Lumbar Vertebrae

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Food Science

Cite this

Smith, B. J., King, J. B., Lucas, E. A., Akhter, M. P., Arjmandi, B. H., & Stoecker, B. J. (2002). Skeletal unloading and dietary copper depletion are detrimental to bone quality of mature rats. Journal of Nutrition, 132(2), 190-196.

Skeletal unloading and dietary copper depletion are detrimental to bone quality of mature rats. / Smith, Brenda J.; King, Jarrod B.; Lucas, Edralin A.; Akhter, Mohammed P.; Arjmandi, Bahram H.; Stoecker, Barbara J.

In: Journal of Nutrition, Vol. 132, No. 2, 2002, p. 190-196.

Research output: Contribution to journalArticle

Smith, BJ, King, JB, Lucas, EA, Akhter, MP, Arjmandi, BH & Stoecker, BJ 2002, 'Skeletal unloading and dietary copper depletion are detrimental to bone quality of mature rats', Journal of Nutrition, vol. 132, no. 2, pp. 190-196.
Smith, Brenda J. ; King, Jarrod B. ; Lucas, Edralin A. ; Akhter, Mohammed P. ; Arjmandi, Bahram H. ; Stoecker, Barbara J. / Skeletal unloading and dietary copper depletion are detrimental to bone quality of mature rats. In: Journal of Nutrition. 2002 ; Vol. 132, No. 2. pp. 190-196.
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