Smoke-induced signal molecules in bone marrow cells from altered low-density lipoprotein receptor-related protein 5 mice

Danjun Ma, Yan Li, Bryan Hackfort, Yingchun Zhao, Jing Xiao, Patrick Swanson, Joan M. Lappe, Peng Xiao, Diane Cullen, Mohammed P. Akhter, Robert R. Recker, Gary Guishan Xiao

Research output: Contribution to journalArticle

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Abstract

Mechanism underlying smoke-induced loss of bone mass is unknown. In this study, we hypothesized that protein signals induced by smoking in bone marrow may be associated with the loss of bone mass. Using a proteomics approach, we identified 38 proteins differentially expressed in bone marrow cells from low-density lipoprotein receptor-related protein 5 (Lrp5) mice exposed to cigarette smoking. Smoking effects on protein expression in bone marrow among three genotypes (Lrp5+/+, Lrp5G171V, and Lrp5 -/-) varied. On the basis of the ratio of protein expression induced by smoking versus nonsmoking, smoke induced protein expression significantly in wild-type mice compared to the other two genotypes (Lrp5G171V and Lrp5-/-). These proteins include inhibitors of β-catenin and proteins associated with differentiation of osteoclasts. We observed that S100A8 and S100A9 were overexpressed in human smokers compared to nonsmokers, which confirmed the effect of smoking on the expression of two proteins in Lrp5 mice, suggesting the role of these proteins in bone remodeling. Smoke induced expression of S100A8 and S100A9 in a time-dependent fashion, which was opposite of the changes in the ratio of OPG/RANKL in bone marrow cells, suggesting that the high levels of S100A8 and S100A9 may be associated with smoke-induced bone loss by increasing bone resorption.

Original languageEnglish
Pages (from-to)3548-3560
Number of pages13
JournalJournal of Proteome Research
Volume11
Issue number7
DOIs
StatePublished - Jul 6 2012

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Smoke
Bone Marrow Cells
Low Density Lipoprotein Receptor-Related Protein-5
Bone
Cells
Molecules
Smoking
Proteins
Bone and Bones
Bone Marrow
Genotype
Catenins
Mouse Lrp5 protein
Bone Remodeling
Osteoclasts
Bone Resorption
Proteomics
Tobacco Products
Cell Count

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

Smoke-induced signal molecules in bone marrow cells from altered low-density lipoprotein receptor-related protein 5 mice. / Ma, Danjun; Li, Yan; Hackfort, Bryan; Zhao, Yingchun; Xiao, Jing; Swanson, Patrick; Lappe, Joan M.; Xiao, Peng; Cullen, Diane; Akhter, Mohammed P.; Recker, Robert R.; Xiao, Gary Guishan.

In: Journal of Proteome Research, Vol. 11, No. 7, 06.07.2012, p. 3548-3560.

Research output: Contribution to journalArticle

Ma, Danjun ; Li, Yan ; Hackfort, Bryan ; Zhao, Yingchun ; Xiao, Jing ; Swanson, Patrick ; Lappe, Joan M. ; Xiao, Peng ; Cullen, Diane ; Akhter, Mohammed P. ; Recker, Robert R. ; Xiao, Gary Guishan. / Smoke-induced signal molecules in bone marrow cells from altered low-density lipoprotein receptor-related protein 5 mice. In: Journal of Proteome Research. 2012 ; Vol. 11, No. 7. pp. 3548-3560.
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