Abstract
A range of extrinsic signals, including afferent activity, affect neuronal growth and plasticity. Neuronal activity regulates intracellular Ca 2+, and activity-dependent calcium signaling has been shown to regulate dendritic growth and branching (Konur and Ghosh, 2005). NMDA receptor (NMDAR) stimulation of Ca 2+/calmodulin-dependent protein kinase signaling cascades has, moreover, been demonstrated to regulate neurite/axonal outgrowth (Wayman et al., 2004). We used a sodium channel activator, brevetoxin (PbTx-2), to explore the relationship between intracellular [Na +] and NMDAR-dependent development. PbTx-2 alone, at a concentration of 30 nM, did not affect Ca 2+ dynamics in 2 d in vitro cerebrocortical neurons; however, this treatment robustly potentiated NMDA-induced Ca 2+ influx. The 30 nM PbTx-2 treatment produced a maximum [Na +] i of 16.9 ±1.5 mM, representing an increment of 8.8 ± 1.8 mM over basal. The corresponding membrane potential change produced by 30 nM PbTx-2 was modest and, therefore, insufficient to relieve the voltage-dependent Mg 2+ block of NMDARs. To unambiguously demonstrate the enhancement of NMDA receptor function by PbTx-2, we recorded single-channel currents from cell-attached patches. PbTx-2 treatment was found to increase both the mean open time and open probability of NMDA receptors. These effects of PbTx-2 on NMDA receptor function were dependent on extracellular Na + and activation of Src kinase. The functional consequences of PbTx-2-induced enhancement of NMDAR function were evaluated in immature cerebrocortical neurons. PbTx-2 concentrations between 3 and 300 nM enhanced neurite outgrowth. Voltage-gated sodium channel activators may accordingly represent a novel pharmacologic strategy to regulate neuronal plasticity through an NMDA receptor and Src family kinase-dependent mechanism.
| Original language | English |
|---|---|
| Pages (from-to) | 3288-3301 |
| Number of pages | 14 |
| Journal | Journal of Neuroscience |
| Volume | 29 |
| Issue number | 10 |
| DOIs | |
| State | Published - Mar 11 2009 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Neuroscience(all)
Cite this
Sodium channel activation augments NMDA receptor function and promotes neurite outgrowth in immature cerebrocortical neurons. / George, Joju; Dravid, Shashank M.; Prakash, Anand; Xie, Jun; Peterson, Jennifer; Jabba, Sairam V.; Baden, Daniel G.; Murray, Thomas F.
In: Journal of Neuroscience, Vol. 29, No. 10, 11.03.2009, p. 3288-3301.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sodium channel activation augments NMDA receptor function and promotes neurite outgrowth in immature cerebrocortical neurons
AU - George, Joju
AU - Dravid, Shashank M.
AU - Prakash, Anand
AU - Xie, Jun
AU - Peterson, Jennifer
AU - Jabba, Sairam V.
AU - Baden, Daniel G.
AU - Murray, Thomas F.
PY - 2009/3/11
Y1 - 2009/3/11
N2 - A range of extrinsic signals, including afferent activity, affect neuronal growth and plasticity. Neuronal activity regulates intracellular Ca 2+, and activity-dependent calcium signaling has been shown to regulate dendritic growth and branching (Konur and Ghosh, 2005). NMDA receptor (NMDAR) stimulation of Ca 2+/calmodulin-dependent protein kinase signaling cascades has, moreover, been demonstrated to regulate neurite/axonal outgrowth (Wayman et al., 2004). We used a sodium channel activator, brevetoxin (PbTx-2), to explore the relationship between intracellular [Na +] and NMDAR-dependent development. PbTx-2 alone, at a concentration of 30 nM, did not affect Ca 2+ dynamics in 2 d in vitro cerebrocortical neurons; however, this treatment robustly potentiated NMDA-induced Ca 2+ influx. The 30 nM PbTx-2 treatment produced a maximum [Na +] i of 16.9 ±1.5 mM, representing an increment of 8.8 ± 1.8 mM over basal. The corresponding membrane potential change produced by 30 nM PbTx-2 was modest and, therefore, insufficient to relieve the voltage-dependent Mg 2+ block of NMDARs. To unambiguously demonstrate the enhancement of NMDA receptor function by PbTx-2, we recorded single-channel currents from cell-attached patches. PbTx-2 treatment was found to increase both the mean open time and open probability of NMDA receptors. These effects of PbTx-2 on NMDA receptor function were dependent on extracellular Na + and activation of Src kinase. The functional consequences of PbTx-2-induced enhancement of NMDAR function were evaluated in immature cerebrocortical neurons. PbTx-2 concentrations between 3 and 300 nM enhanced neurite outgrowth. Voltage-gated sodium channel activators may accordingly represent a novel pharmacologic strategy to regulate neuronal plasticity through an NMDA receptor and Src family kinase-dependent mechanism.
AB - A range of extrinsic signals, including afferent activity, affect neuronal growth and plasticity. Neuronal activity regulates intracellular Ca 2+, and activity-dependent calcium signaling has been shown to regulate dendritic growth and branching (Konur and Ghosh, 2005). NMDA receptor (NMDAR) stimulation of Ca 2+/calmodulin-dependent protein kinase signaling cascades has, moreover, been demonstrated to regulate neurite/axonal outgrowth (Wayman et al., 2004). We used a sodium channel activator, brevetoxin (PbTx-2), to explore the relationship between intracellular [Na +] and NMDAR-dependent development. PbTx-2 alone, at a concentration of 30 nM, did not affect Ca 2+ dynamics in 2 d in vitro cerebrocortical neurons; however, this treatment robustly potentiated NMDA-induced Ca 2+ influx. The 30 nM PbTx-2 treatment produced a maximum [Na +] i of 16.9 ±1.5 mM, representing an increment of 8.8 ± 1.8 mM over basal. The corresponding membrane potential change produced by 30 nM PbTx-2 was modest and, therefore, insufficient to relieve the voltage-dependent Mg 2+ block of NMDARs. To unambiguously demonstrate the enhancement of NMDA receptor function by PbTx-2, we recorded single-channel currents from cell-attached patches. PbTx-2 treatment was found to increase both the mean open time and open probability of NMDA receptors. These effects of PbTx-2 on NMDA receptor function were dependent on extracellular Na + and activation of Src kinase. The functional consequences of PbTx-2-induced enhancement of NMDAR function were evaluated in immature cerebrocortical neurons. PbTx-2 concentrations between 3 and 300 nM enhanced neurite outgrowth. Voltage-gated sodium channel activators may accordingly represent a novel pharmacologic strategy to regulate neuronal plasticity through an NMDA receptor and Src family kinase-dependent mechanism.
UR - http://www.scopus.com/inward/record.url?scp=63849305126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63849305126&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.6104-08.2009
DO - 10.1523/JNEUROSCI.6104-08.2009
M3 - Article
C2 - 19279266
AN - SCOPUS:63849305126
VL - 29
SP - 3288
EP - 3301
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 10
ER -