Solid phase synthesis and evaluation of Tyr-Tic-Phe-Phe(p-NHCOCH2Br) ([Phe(p-bromoacetamide)4]TIPP), a potent affinity label for δ opioid receptors

Vivek Kumar; Thomas F. Murray; Jane V. Aldrich

doi:10.1021/jm020290p

Solid phase synthesis and evaluation of Tyr-Tic-Phe-Phe(p-NHCOCH₂Br) ([Phe(p-bromoacetamide)⁴]TIPP), a potent affinity label for δ opioid receptors

Vivek Kumar, Thomas F. Murray, Jane V. Aldrich

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Derivatives of the δ opioid receptor selective peptide Tyr-Tic-Phe-Phe-OH (TIPP) containing a p-bromoacetamide moiety on the phenyl ring of Phe³ or Phe⁴ were prepared by solid phase synthesis. [Phe(p-NHCOCH₂Br)⁴]TIPP exhibited high affinity for cloned δ receptors (IC₅₀ = 5.4 nM), and incubation with only 2.5 nM resulted in 85% wash resistant inhibition of radioligand binding to δ receptors. Therefore, this peptide is a potent affinity label for further study of δ opioid receptors.

Original language	English (US)
Pages (from-to)	3820-3823
Number of pages	4
Journal	Journal of Medicinal Chemistry
Volume	45
Issue number	18
DOIs	https://doi.org/10.1021/jm020290p
State	Published - Aug 29 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Medicine
Drug Discovery

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title = "Solid phase synthesis and evaluation of Tyr-Tic-Phe-Phe(p-NHCOCH2Br) ([Phe(p-bromoacetamide)4]TIPP), a potent affinity label for δ opioid receptors",

abstract = "Derivatives of the δ opioid receptor selective peptide Tyr-Tic-Phe-Phe-OH (TIPP) containing a p-bromoacetamide moiety on the phenyl ring of Phe3 or Phe4 were prepared by solid phase synthesis. [Phe(p-NHCOCH2Br)4]TIPP exhibited high affinity for cloned δ receptors (IC50 = 5.4 nM), and incubation with only 2.5 nM resulted in 85% wash resistant inhibition of radioligand binding to δ receptors. Therefore, this peptide is a potent affinity label for further study of δ opioid receptors.",

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AU - Murray, Thomas F.

AU - Aldrich, Jane V.

PY - 2002/8/29

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N2 - Derivatives of the δ opioid receptor selective peptide Tyr-Tic-Phe-Phe-OH (TIPP) containing a p-bromoacetamide moiety on the phenyl ring of Phe3 or Phe4 were prepared by solid phase synthesis. [Phe(p-NHCOCH2Br)4]TIPP exhibited high affinity for cloned δ receptors (IC50 = 5.4 nM), and incubation with only 2.5 nM resulted in 85% wash resistant inhibition of radioligand binding to δ receptors. Therefore, this peptide is a potent affinity label for further study of δ opioid receptors.

AB - Derivatives of the δ opioid receptor selective peptide Tyr-Tic-Phe-Phe-OH (TIPP) containing a p-bromoacetamide moiety on the phenyl ring of Phe3 or Phe4 were prepared by solid phase synthesis. [Phe(p-NHCOCH2Br)4]TIPP exhibited high affinity for cloned δ receptors (IC50 = 5.4 nM), and incubation with only 2.5 nM resulted in 85% wash resistant inhibition of radioligand binding to δ receptors. Therefore, this peptide is a potent affinity label for further study of δ opioid receptors.

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