Specification of germ cell fates by FOG-3 has been conserved during nematode evolution

P. J. Chen, Soochin Cho, S. W. Jin, R. E. Ellis

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Rapid changes in sexual traits are ubiquitous in evolution. To analyze this phenomenon, we are studying species of the genus Caenorhabditis. These animals use one of two different mating systems - male/hermaphroditic, like the model organism Caenorhabditis elegans, or male/female, like C. remanei. Since hermaphrodites are essentially females that produce sperm for self-fertilization, elucidating the control of cell fate in the germ line in each species could provide the key to understanding how these mating systems evolved. In C. elegans, FOG-3 is required to specify that germ cells become sperm. Thus, we cloned its homologs from both C. remanei and C. briggsae. Each species produces a single homolog of FOG-3, and RNA-mediated interference indicates that FOG-3 functions in each species to specify that germ cells develop as sperm rather than as oocytes. What factors account for the different mating systems? Northern analyses and RT-PCR data reveal that the expression of fog-3 is always correlated with spermatogenesis. Since the promoters for all three fog-3 genes contain binding sites for the transcription factor TRA-1A and are capable of driving expression of fog-3 in C. elegans hermaphrodites, we propose that alterations in the upstream sex-determination pathway, perhaps acting through TRA-1A, allow spermatogenesis in C. elegans and C. briggsae XX larvae but not in C. remanei.

Original languageEnglish
Pages (from-to)1513-1525
Number of pages13
JournalGenetics
Volume158
Issue number4
StatePublished - 2001
Externally publishedYes

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Caenorhabditis elegans
Germ Cells
Weather
Spermatozoa
Spermatogenesis
Caenorhabditis
Self-Fertilization
RNA Interference
Oocytes
Larva
Transcription Factors
Binding Sites
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Chen, P. J., Cho, S., Jin, S. W., & Ellis, R. E. (2001). Specification of germ cell fates by FOG-3 has been conserved during nematode evolution. Genetics, 158(4), 1513-1525.

Specification of germ cell fates by FOG-3 has been conserved during nematode evolution. / Chen, P. J.; Cho, Soochin; Jin, S. W.; Ellis, R. E.

In: Genetics, Vol. 158, No. 4, 2001, p. 1513-1525.

Research output: Contribution to journalArticle

Chen, PJ, Cho, S, Jin, SW & Ellis, RE 2001, 'Specification of germ cell fates by FOG-3 has been conserved during nematode evolution', Genetics, vol. 158, no. 4, pp. 1513-1525.
Chen, P. J. ; Cho, Soochin ; Jin, S. W. ; Ellis, R. E. / Specification of germ cell fates by FOG-3 has been conserved during nematode evolution. In: Genetics. 2001 ; Vol. 158, No. 4. pp. 1513-1525.
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