Spermine detection via metal-mediated ethynylarene 'turn-on' fluorescence signaling

James T. Fletcher, Brent S. Bruck

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A dicarboxylated ethynylarene was shown to behave as a fluorescent chemosensor for millimolar concentrations of polyamines when mixed with Cd(II), Pb(II) or Zn(II) ions at micromolar concentrations. A bathochromic shift and intensification of fluorescence emission was observed with increasing amounts of metal ion in the presence of aqueous polyamines buffered at pH = 7.6. Such perturbations manifested as 'turn-on' signals from a ratiometric comparison of emission intensities at 390 nm versus 340 nm. Using Pb(II) as the metal mediator, spermine was selectively detected as a 40-fold signal enhancement relative to spermidine, putrescine, cadaverine and several other non-biogenic diamines. Evaluation of additional triamine and tetraamine analytes showed the influence that amine group quantity and spacing had on signal generation. By increasing the ratio of Pb(II) relative to ethynylarene, the detection limit for spermine was successfully lowered to a 25 micromolar level. Noncovalent association between ethynylarene, metal ion and polyamine are believed to promote the observed spectroscopic changes. This study exploits the subtle impact that polyamine structural identity has on transition metal chelation to define a new approach toward polyamine chemosensor development.

Original languageEnglish
Pages (from-to)843-848
Number of pages6
JournalSensors and Actuators, B: Chemical
Issue numberPartA
DOIs
StatePublished - Aug 9 2015

Fingerprint

Spermine
Polyamines
Metals
Fluorescence
fluorescence
metal ions
metals
chelation
Metal ions
diamines
Cadaverine
amines
transition metals
spacing
Diamines
Putrescine
Spermidine
perturbation
Chelation
augmentation

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Instrumentation

Cite this

Spermine detection via metal-mediated ethynylarene 'turn-on' fluorescence signaling. / Fletcher, James T.; Bruck, Brent S.

In: Sensors and Actuators, B: Chemical, No. PartA, 09.08.2015, p. 843-848.

Research output: Contribution to journalArticle

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