Splits ends is a tissue/promoter specific regulator of Wingless signaling

Hua V. Lin, David B. Doroquez, Soochin Cho, Fangli Chen, Ilaria Rebay, Ken M. Cadigan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Wingless directs many developmental processes in Drosophila by regulating expression of specific target genes through a conserved signaling pathway. Although many nuclear factors have been implicated in mediating Wingless-induced transcription, the mechanism of how Wingless regulates different targets in different tissues remains poorly understood. We report here that the split ends gene is required for Wingless signaling in the eye, wing and leg imaginal discs. Expression of a dominant-negative version of split ends resulted in more dramatic reductions in Wingless signaling than split ends-null alleles, suggesting that it may have a redundant partner. However, removal of split ends or expression of the dominant-negative had no effect on several Wingless signaling readouts in the embryo. The expression pattern of Split ends cannot explain this tissue-specific requirement, as the protein is predominantly nuclear and present throughout embryogenesis and larval tissues. Consistent with its nuclear location, the split ends dominant-negative acts downstream of Armadillo stabilization. Our data indicate that Split ends is an important positive regulator of Wingless signaling in larval tissues. However, it has no detectable role in the embryonic Wingless pathway, suggesting that it is a tissue or promoter-specific factor.

Original languageEnglish
Pages (from-to)3125-3135
Number of pages11
JournalDevelopment
Volume130
Issue number14
StatePublished - Jul 2003
Externally publishedYes

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Armadillos
Imaginal Discs
Genes
Drosophila
Embryonic Development
Leg
Embryonic Structures
Alleles
Proteins

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Cell Biology

Cite this

Lin, H. V., Doroquez, D. B., Cho, S., Chen, F., Rebay, I., & Cadigan, K. M. (2003). Splits ends is a tissue/promoter specific regulator of Wingless signaling. Development, 130(14), 3125-3135.

Splits ends is a tissue/promoter specific regulator of Wingless signaling. / Lin, Hua V.; Doroquez, David B.; Cho, Soochin; Chen, Fangli; Rebay, Ilaria; Cadigan, Ken M.

In: Development, Vol. 130, No. 14, 07.2003, p. 3125-3135.

Research output: Contribution to journalArticle

Lin, HV, Doroquez, DB, Cho, S, Chen, F, Rebay, I & Cadigan, KM 2003, 'Splits ends is a tissue/promoter specific regulator of Wingless signaling', Development, vol. 130, no. 14, pp. 3125-3135.
Lin HV, Doroquez DB, Cho S, Chen F, Rebay I, Cadigan KM. Splits ends is a tissue/promoter specific regulator of Wingless signaling. Development. 2003 Jul;130(14):3125-3135.
Lin, Hua V. ; Doroquez, David B. ; Cho, Soochin ; Chen, Fangli ; Rebay, Ilaria ; Cadigan, Ken M. / Splits ends is a tissue/promoter specific regulator of Wingless signaling. In: Development. 2003 ; Vol. 130, No. 14. pp. 3125-3135.
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