Sry-type HMG box 18 contributes to the differentiation of bone marrow-derived mesenchymal stem cells to endothelial cells

Izuagie Attairu Ikhapoh, Christopher J. Pelham, Devendra K. Agrawal

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: Mesenchymal stem cells (MSC) have shown therapeutic potential to engraft and either differentiate into or support differentiation of vascular endothelial cells (EC), smooth muscle cells and cardiomyocytes in animal models of ischemic heart disease. Following intracoronary or transendocardial delivery of MSCs, however, only a small fraction of cells engraft and the majority of those persist as an immature cell phenotype. The goal of the current study was to decipher the molecular pathways and mechanisms that control MSC differentiation into ECs. Vascular endothelial growth factor (VEGF-165) treatment is known to enhance in vitro differentiation of MSCs into ECs. We tested the possible involvement of the Sry-type HMG box (Sox) family of transcription factors in this process. Method and Results: MSCs were isolated from the bone marrow of Yucatan microswine and underwent a 10 day differentiation protocol. VEGF-165 (50. ng/ml) treatment of MSCs in vitro induced a significant increase in the protein expression of VEGFR-2, Sox9 and Sox18, in addition to the EC markers PECAM-1, VE-cadherin and vWF, as determined by Western blot or flow cytometry. siRNA-mediated knockdown of Sox18, as opposed to Sox9, in MSCs prevented VEGF-165-mediated induction of EC markers and capillary tube formation. Inhibition of VEGFR-2 signaling (SC-202850) reduced Sox18 and reduced VEGF-165-induced differentiation of MSCs to ECs. Conclusion: Here we demonstrate that VEGF-165 mediates MSC differentiation into ECs via VEGFR-2-dependent induction of Sox18, which ultimately coordinates the transcriptional upregulation of specific markers of the EC phenotype.

Original languageEnglish
Pages (from-to)87-96
Number of pages10
JournalDifferentiation
Volume89
Issue number3-4
DOIs
StatePublished - Sep 29 2014

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Mesenchymal Stromal Cells
Endothelial Cells
Bone Marrow
Vascular Endothelial Growth Factor Receptor-2
Cell Differentiation
CD31 Antigens
Phenotype
Cardiac Myocytes
Small Interfering RNA
Smooth Muscle Myocytes
Myocardial Ischemia
human VEGFA protein
Flow Cytometry
Transcription Factors
Up-Regulation
Animal Models
Western Blotting
Proteins

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology
  • Molecular Biology
  • Cancer Research

Cite this

Sry-type HMG box 18 contributes to the differentiation of bone marrow-derived mesenchymal stem cells to endothelial cells. / Ikhapoh, Izuagie Attairu; Pelham, Christopher J.; Agrawal, Devendra K.

In: Differentiation, Vol. 89, No. 3-4, 29.09.2014, p. 87-96.

Research output: Contribution to journalArticle

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