Statherin is an in vivo pellicle constituent

Identification and immuno-quantification

J. Li, E. J. Helmerhorst, Y. Yao, Martha E. Nunn, R. F. Troxler, F. G. Oppenheim

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Recently, we demonstrated that anti-statherin monoclonal antibodies could be generated upon immunisation of mice with in vivo formed human acquired enamel pellicle, indicating that statherin is a constituent of pellicle. To gain insight in the in vivo adsorption behaviour of statherin we tested the abundance of statherin in pellicle and investigated the relationship between statherin and protein levels in salivary secretions and pellicle using a capture ELISA. Statherin levels were approximately 20-fold higher in parotid and submandibular-sublingual secretions than in cleared whole saliva supernatant or pellicle, suggesting the rapid degradation of statherin in the oral cavity. A strong positive correlation was observed between statherin and protein levels in pellicle but not in saliva indicating that statherin and protein adsorption to pellicle are related processes. This indicates that statherin represents the integral part of proteins that constitute the pellicle structure and may play a key role in its formation.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalArchives of Oral Biology
Volume49
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

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Dental Pellicle
Saliva
Adsorption
Proteins
Mouth
Immunization
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Statherin is an in vivo pellicle constituent : Identification and immuno-quantification. / Li, J.; Helmerhorst, E. J.; Yao, Y.; Nunn, Martha E.; Troxler, R. F.; Oppenheim, F. G.

In: Archives of Oral Biology, Vol. 49, No. 5, 05.2004, p. 379-385.

Research output: Contribution to journalArticle

Li, J. ; Helmerhorst, E. J. ; Yao, Y. ; Nunn, Martha E. ; Troxler, R. F. ; Oppenheim, F. G. / Statherin is an in vivo pellicle constituent : Identification and immuno-quantification. In: Archives of Oral Biology. 2004 ; Vol. 49, No. 5. pp. 379-385.
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