Stereoselective L-[3H]quinuclidinyl benzilate-binding sites in nervous tissue of Aplysia californica

Evidence for muscarinic receptors

Thomas F. Murray, G. Mpitsos, J. F. Siebenaller, D. L. Barkers

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The muscarinic antagonist L-[3H]quinuclidinyl benzilate (L-[3H]QNB) binds with a high affinity (K(d) = 0.77 nm) to a single population of specific sites (B(max) = 47 fmol/mg of protein) in nervous tissue of the gastropod mullusc, Aplysia. The specific L-[3H]QNB binding is displaced stereoselectively by the enantiomers of benzetimide, dexetimide,and levetimide. The pharmacologically active enantiomer, dexetimide, is more potent than levetimide as an inhibitor of L-[3H]QNB binding. Moreover, the muscarinic cholinergic ligands, scopolamine, atropine, oxotremorine, and pilocarpine are effective inhibitors of the specific L-[3H]QNB binding, whereas nicotinic receptor antagonists, decamethonium and d-tubocurarine, are considerably less effective. These pharmacological characteristics of the L-[3H]QNB-binding site provide evidence for classical muscarinic receptors in Aplysia nervous tissue. The physiological relevance of the dexetimide-displaceable L-[3H]QNB-binding site was supported by the demonstration of the sensitivity of the specific binding to thermal denaturation. Specific binding of L-[3H]QNB was also detected in nervous tissue of another marine gastropod, Pleurobranchaea californica. The characteristics of the Aplysia L-[3H]QNB-binding site are in accordance with studies of numerous vertebrate and invertebrate tissues indicating that the muscarinic cholinergic receptor site has been highly conserved through evolution.

Original languageEnglish
Pages (from-to)3184-3188
Number of pages5
JournalJournal of Neuroscience
Volume5
Issue number12
StatePublished - 1985
Externally publishedYes

Fingerprint

Quinuclidinyl Benzilate
Dexetimide
Nerve Tissue
Aplysia
Muscarinic Receptors
Gastropoda
Cholinergic Agents
Pleurobranchaea
Nicotinic Antagonists
Oxotremorine
Tubocurarine
Pilocarpine
Muscarinic Antagonists
Scopolamine Hydrobromide
Nicotinic Receptors
Cholinergic Receptors
Invertebrates
Atropine
Vertebrates
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Stereoselective L-[3H]quinuclidinyl benzilate-binding sites in nervous tissue of Aplysia californica : Evidence for muscarinic receptors. / Murray, Thomas F.; Mpitsos, G.; Siebenaller, J. F.; Barkers, D. L.

In: Journal of Neuroscience, Vol. 5, No. 12, 1985, p. 3184-3188.

Research output: Contribution to journalArticle

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