Abstract
The effect of Escherichia coli heat-stable (ST) enterotoxin on calcium and cyclic nucleotide metabolism in rat basophilic leukemia cell cultures was investigated. Addition of ST enterotoxin to rat basophilic leukemia cell cultures resulted in dose- and time-dependent stimulation of calcium uptake and elevation of the intracellular cyclic GMP (cGMP) concentration. The effect of ST enterotoxin on calcium uptake (P < 0.02) and cGMP synthesis (P < 0.02) was demonstrated after 5 and 30 min of incubation at 37°C, respectively. In further studies ST enterotoxin did not enhance calcium release or the intracellular concentraton of cyclic AMP. The stimulation of calcium uptake and cGMP synthesis by ST enterotoxin was inhibited by pharmacological and chemical agents which block cellular calcium entry and prostaglandin synthesis. These results demonstrate that ST enterotoxin induces calcium uptake and cGMP synthesis in rat basophilic leukemia cell cultures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 971-977 |
| Number of pages | 7 |
| Journal | Infection and Immunity |
| Volume | 41 |
| Issue number | 3 |
| State | Published - Jan 1 1983 |
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All Science Journal Classification (ASJC) codes
- Immunology
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Stimulation of calcium uptake and cyclic GMP synthesis in rat basophilic leukemia cells by Escherichia coli heat-stable enterotoxin. / Knoop, Floyd C.; Thomas, D. D.
In: Infection and Immunity, Vol. 41, No. 3, 01.01.1983, p. 971-977.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Stimulation of calcium uptake and cyclic GMP synthesis in rat basophilic leukemia cells by Escherichia coli heat-stable enterotoxin
AU - Knoop, Floyd C.
AU - Thomas, D. D.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - The effect of Escherichia coli heat-stable (ST) enterotoxin on calcium and cyclic nucleotide metabolism in rat basophilic leukemia cell cultures was investigated. Addition of ST enterotoxin to rat basophilic leukemia cell cultures resulted in dose- and time-dependent stimulation of calcium uptake and elevation of the intracellular cyclic GMP (cGMP) concentration. The effect of ST enterotoxin on calcium uptake (P < 0.02) and cGMP synthesis (P < 0.02) was demonstrated after 5 and 30 min of incubation at 37°C, respectively. In further studies ST enterotoxin did not enhance calcium release or the intracellular concentraton of cyclic AMP. The stimulation of calcium uptake and cGMP synthesis by ST enterotoxin was inhibited by pharmacological and chemical agents which block cellular calcium entry and prostaglandin synthesis. These results demonstrate that ST enterotoxin induces calcium uptake and cGMP synthesis in rat basophilic leukemia cell cultures.
AB - The effect of Escherichia coli heat-stable (ST) enterotoxin on calcium and cyclic nucleotide metabolism in rat basophilic leukemia cell cultures was investigated. Addition of ST enterotoxin to rat basophilic leukemia cell cultures resulted in dose- and time-dependent stimulation of calcium uptake and elevation of the intracellular cyclic GMP (cGMP) concentration. The effect of ST enterotoxin on calcium uptake (P < 0.02) and cGMP synthesis (P < 0.02) was demonstrated after 5 and 30 min of incubation at 37°C, respectively. In further studies ST enterotoxin did not enhance calcium release or the intracellular concentraton of cyclic AMP. The stimulation of calcium uptake and cGMP synthesis by ST enterotoxin was inhibited by pharmacological and chemical agents which block cellular calcium entry and prostaglandin synthesis. These results demonstrate that ST enterotoxin induces calcium uptake and cGMP synthesis in rat basophilic leukemia cell cultures.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020591937&partnerID=8YFLogxK
M3 - Article
VL - 41
SP - 971
EP - 977
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 3
ER -