Streamlined procedure for the production of normal and altered versions of recombinant human proinsulin

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10 Citations (Scopus)

Abstract

A method for the simplified, reproducible production of both normal and altered versions of human proinsulin has been developed. A polyhistidine/proinsulin fusion protein was expressed using a prokaryotic expression system and partially purified by affinity chromatography. Disulfide bonds within the polypeptide were formed prior to removal of the affinity tag. The proinsulin cleaved from the fusion protein was then subjected to a final purification step of semipreparative reversed-phase high-performance liquid chromatography. Integrity of both the normal and mutant proinsulins was confirmed by peptide mapping and mass spectrometry. The different versions of proinsulin will be used to map those residues of the substrate used in cleavage site recognition by members of the furin/PC family of converting enzymes.

Original languageEnglish
Pages (from-to)308-313
Number of pages6
JournalProtein Expression and Purification
Volume15
Issue number3
DOIs
StatePublished - 1999

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Proinsulin
Fusion reactions
Furin
Affinity chromatography
Peptides
Peptide Mapping
High performance liquid chromatography
Reverse-Phase Chromatography
Affinity Chromatography
Disulfides
Purification
Mass spectrometry
Mass Spectrometry
Proteins
High Pressure Liquid Chromatography
Substrates
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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AB - A method for the simplified, reproducible production of both normal and altered versions of human proinsulin has been developed. A polyhistidine/proinsulin fusion protein was expressed using a prokaryotic expression system and partially purified by affinity chromatography. Disulfide bonds within the polypeptide were formed prior to removal of the affinity tag. The proinsulin cleaved from the fusion protein was then subjected to a final purification step of semipreparative reversed-phase high-performance liquid chromatography. Integrity of both the normal and mutant proinsulins was confirmed by peptide mapping and mass spectrometry. The different versions of proinsulin will be used to map those residues of the substrate used in cleavage site recognition by members of the furin/PC family of converting enzymes.

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