Streptococcus agalactiae strains with chromosomal deletions evade detection with molecular methods

Isabella A. Tickler, Fred C. Tenover, Scott Dewell, Victoria M. Le, Rachel N. Blackman, Richard V. Goering, Amy E. Rogers, Heather Piwonka, Brittney D. Jung-Hynes, Derrick J. Chen, Michael J. Loeffelholz, Devasena Gnanashanmugam, Ellen Jo Baron

Research output: Contribution to journalArticle

Abstract

Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culturepositive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample was <1% in three geographic locations but 7% in a fourth location. Among the 15 isolates with chromosomal deletions, multiple pulsed-field gel electrophoresis types were identified, one of which appears to be broadly dispersed across the United States.

Original languageEnglish (US)
Article numbere02040-18
JournalJournal of clinical microbiology
Volume57
Issue number4
DOIs
StatePublished - Apr 1 2018

Fingerprint

Streptococcus agalactiae
Geographic Locations
Streptococcus
Hemolysin Proteins
Molecular Pathology
Pulsed Field Gel Electrophoresis
Ireland
Routine Diagnostic Tests
Chromosomes
Genome
Prospective Studies
Polymerase Chain Reaction
DNA
Population
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Streptococcus agalactiae strains with chromosomal deletions evade detection with molecular methods. / Tickler, Isabella A.; Tenover, Fred C.; Dewell, Scott; Le, Victoria M.; Blackman, Rachel N.; Goering, Richard V.; Rogers, Amy E.; Piwonka, Heather; Jung-Hynes, Brittney D.; Chen, Derrick J.; Loeffelholz, Michael J.; Gnanashanmugam, Devasena; Baron, Ellen Jo.

In: Journal of clinical microbiology, Vol. 57, No. 4, e02040-18, 01.04.2018.

Research output: Contribution to journalArticle

Tickler, IA, Tenover, FC, Dewell, S, Le, VM, Blackman, RN, Goering, RV, Rogers, AE, Piwonka, H, Jung-Hynes, BD, Chen, DJ, Loeffelholz, MJ, Gnanashanmugam, D & Baron, EJ 2018, 'Streptococcus agalactiae strains with chromosomal deletions evade detection with molecular methods', Journal of clinical microbiology, vol. 57, no. 4, e02040-18. https://doi.org/10.1128/JCM.02040-18_rfseq1
Tickler, Isabella A. ; Tenover, Fred C. ; Dewell, Scott ; Le, Victoria M. ; Blackman, Rachel N. ; Goering, Richard V. ; Rogers, Amy E. ; Piwonka, Heather ; Jung-Hynes, Brittney D. ; Chen, Derrick J. ; Loeffelholz, Michael J. ; Gnanashanmugam, Devasena ; Baron, Ellen Jo. / Streptococcus agalactiae strains with chromosomal deletions evade detection with molecular methods. In: Journal of clinical microbiology. 2018 ; Vol. 57, No. 4.
@article{4897b7c276964698a49d9e526fba8f64,
title = "Streptococcus agalactiae strains with chromosomal deletions evade detection with molecular methods",
abstract = "Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culturepositive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample was <1{\%} in three geographic locations but 7{\%} in a fourth location. Among the 15 isolates with chromosomal deletions, multiple pulsed-field gel electrophoresis types were identified, one of which appears to be broadly dispersed across the United States.",
author = "Tickler, {Isabella A.} and Tenover, {Fred C.} and Scott Dewell and Le, {Victoria M.} and Blackman, {Rachel N.} and Goering, {Richard V.} and Rogers, {Amy E.} and Heather Piwonka and Jung-Hynes, {Brittney D.} and Chen, {Derrick J.} and Loeffelholz, {Michael J.} and Devasena Gnanashanmugam and Baron, {Ellen Jo}",
year = "2018",
month = "4",
day = "1",
doi = "10.1128/JCM.02040-18_rfseq1",
language = "English (US)",
volume = "57",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Streptococcus agalactiae strains with chromosomal deletions evade detection with molecular methods

AU - Tickler, Isabella A.

AU - Tenover, Fred C.

AU - Dewell, Scott

AU - Le, Victoria M.

AU - Blackman, Rachel N.

AU - Goering, Richard V.

AU - Rogers, Amy E.

AU - Piwonka, Heather

AU - Jung-Hynes, Brittney D.

AU - Chen, Derrick J.

AU - Loeffelholz, Michael J.

AU - Gnanashanmugam, Devasena

AU - Baron, Ellen Jo

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culturepositive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample was <1% in three geographic locations but 7% in a fourth location. Among the 15 isolates with chromosomal deletions, multiple pulsed-field gel electrophoresis types were identified, one of which appears to be broadly dispersed across the United States.

AB - Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culturepositive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample was <1% in three geographic locations but 7% in a fourth location. Among the 15 isolates with chromosomal deletions, multiple pulsed-field gel electrophoresis types were identified, one of which appears to be broadly dispersed across the United States.

UR - http://www.scopus.com/inward/record.url?scp=85065491624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065491624&partnerID=8YFLogxK

U2 - 10.1128/JCM.02040-18_rfseq1

DO - 10.1128/JCM.02040-18_rfseq1

M3 - Article

AN - SCOPUS:85065491624

VL - 57

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 4

M1 - e02040-18

ER -