TY - JOUR
T1 - Striatal glutamate delta-1 receptor regulates behavioral flexibility and thalamostriatal connectivity
AU - Liu, Jinxu
AU - Shelkar, Gajanan P.
AU - Gandhi, Pauravi J.
AU - Gawande, Dinesh Y.
AU - Hoover, Andrew
AU - Villalba, Rosa M.
AU - Pavuluri, Ratnamala
AU - Smith, Yoland
AU - Dravid, Shashank M.
N1 - Funding Information:
This work was supported by grants from the National Science Foundation #1456818 (SMD), National Institutes of Health NS104705 (SMD), National Institutes of Health MH116003 (SMD) and Yerkes National Primate Center National Institutes of Health/ORIP base grant P51OD11132 (YS). The authors note no conflict of interest.
Funding Information:
This work was supported by grants from the National Science Foundation # 1456818 (SMD), National Institutes of Health NS104705 (SMD), National Institutes of Health MH116003 (SMD) and Yerkes National Primate Center National Institutes of Health/ORIP base grant P51OD11132 (YS). The authors note no conflict of interest.
Publisher Copyright:
© 2020 The Authors
PY - 2020/4
Y1 - 2020/4
N2 - Impaired behavioral flexibility and repetitive behavior is a common phenotype in autism and other neuropsychiatric disorders, but the underlying synaptic mechanisms are poorly understood. The trans-synaptic glutamate delta (GluD)-Cerebellin 1-Neurexin complex, critical for synapse formation/maintenance, represents a vulnerable axis for neuropsychiatric diseases. We have previously found that GluD1 deletion results in reversal learning deficit and repetitive behavior. In this study, we show that selective ablation of GluD1 from the dorsal striatum impairs behavioral flexibility in a water T-maze task. We further found that striatal GluD1 is preferentially found in dendritic shafts, and more frequently associated with thalamic than cortical glutamatergic terminals suggesting localization to projections from the thalamic parafascicular nucleus (Pf). Conditional deletion of GluD1 from the striatum led to a selective loss of thalamic, but not cortical, terminals, and reduced glutamatergic neurotransmission. Optogenetic studies demonstrated functional changes at thalamostriatal synapses from the Pf, but no effect on the corticostriatal system, upon ablation of GluD1 in the dorsal striatum. These studies suggest a novel molecular mechanism by which genetic variations associated with neuropsychiatric disorders may impair behavioral flexibility, and reveal a unique principle by which GluD1 subunit regulates forebrain circuits.
AB - Impaired behavioral flexibility and repetitive behavior is a common phenotype in autism and other neuropsychiatric disorders, but the underlying synaptic mechanisms are poorly understood. The trans-synaptic glutamate delta (GluD)-Cerebellin 1-Neurexin complex, critical for synapse formation/maintenance, represents a vulnerable axis for neuropsychiatric diseases. We have previously found that GluD1 deletion results in reversal learning deficit and repetitive behavior. In this study, we show that selective ablation of GluD1 from the dorsal striatum impairs behavioral flexibility in a water T-maze task. We further found that striatal GluD1 is preferentially found in dendritic shafts, and more frequently associated with thalamic than cortical glutamatergic terminals suggesting localization to projections from the thalamic parafascicular nucleus (Pf). Conditional deletion of GluD1 from the striatum led to a selective loss of thalamic, but not cortical, terminals, and reduced glutamatergic neurotransmission. Optogenetic studies demonstrated functional changes at thalamostriatal synapses from the Pf, but no effect on the corticostriatal system, upon ablation of GluD1 in the dorsal striatum. These studies suggest a novel molecular mechanism by which genetic variations associated with neuropsychiatric disorders may impair behavioral flexibility, and reveal a unique principle by which GluD1 subunit regulates forebrain circuits.
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U2 - 10.1016/j.nbd.2020.104746
DO - 10.1016/j.nbd.2020.104746
M3 - Article
C2 - 31945419
AN - SCOPUS:85078166103
VL - 137
JO - Neurobiology of Disease
JF - Neurobiology of Disease
SN - 0969-9961
M1 - 104746
ER -