TY - JOUR
T1 - Structure and chromosome localization of the human CASP8 gene
AU - Grenet, Jose
AU - Teitz, Tal
AU - Wei, Tie
AU - Valentine, Virginia
AU - Kidd, Vincent J.
N1 - Funding Information:
The authors would like to acknowledge the Molecular Resources Core Facility of SJCRH for oligonucleotide synthesis and automated DNA sequence analysis. This research was supported by a grant from the NIH to V.J.K. (CA 67938), a Cancer Center Core grant from the NIH to SJCRH (CA 21765), and by support from the American Lebanese Syrian Associated Charities (ALSAC).
PY - 1999/1/21
Y1 - 1999/1/21
N2 - The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1β converting enzyme (ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex (DISC). We report here that the CASP8 gene contains at least 11 exons spanning 30 kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8. Chromosome 2 band q33-34 is also involved in tumorigenesis, with loss of heterogeneity (LOH) being reported in a number of tumors. We also report EcoRI and HindIII polymorphisms that may prove to be useful in disease analysis. Both caspases 8 and 10 contain long pro-domains with duplicated death effector domains (DEDs), as well as their corresponding cysteine protease catalytic domains. Thus, it appears that CASP8 and CASP10 have evolved by tandem gene duplication, much like the CASP1, CASP4 and CASP5 gene cluster on human chromosome 11q22.2-22.3.
AB - The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1β converting enzyme (ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex (DISC). We report here that the CASP8 gene contains at least 11 exons spanning 30 kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8. Chromosome 2 band q33-34 is also involved in tumorigenesis, with loss of heterogeneity (LOH) being reported in a number of tumors. We also report EcoRI and HindIII polymorphisms that may prove to be useful in disease analysis. Both caspases 8 and 10 contain long pro-domains with duplicated death effector domains (DEDs), as well as their corresponding cysteine protease catalytic domains. Thus, it appears that CASP8 and CASP10 have evolved by tandem gene duplication, much like the CASP1, CASP4 and CASP5 gene cluster on human chromosome 11q22.2-22.3.
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U2 - 10.1016/S0378-1119(98)00565-4
DO - 10.1016/S0378-1119(98)00565-4
M3 - Article
C2 - 9931493
AN - SCOPUS:0033590598
VL - 226
SP - 225
EP - 232
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -