Study of 3D cell morphology and effect on light scattering distribution

Andrew Ekpenyong, Junhua Ding, Li V. Yang, Nancy R. Leffler, Jun Q. Lu, R. Scott Brock, Xin Hua Hu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.

Original languageEnglish
Title of host publicationAdvanced Microscopy Techniques
Volume7367
DOIs
StatePublished - 2009
Externally publishedYes
EventAdvanced Microscopy Techniques - Munich, Germany
Duration: Jun 14 2009Jun 16 2009

Other

OtherAdvanced Microscopy Techniques
CountryGermany
CityMunich
Period6/14/096/16/09

Fingerprint

Mitochondria
Light scattering
Melanoma
light scattering
Genes
Light
mitochondria
cytoplasm
cells
finite difference time domain method
genes
mice
Cytoplasm
nuclei

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Ekpenyong, A., Ding, J., Yang, L. V., Leffler, N. R., Lu, J. Q., Brock, R. S., & Hu, X. H. (2009). Study of 3D cell morphology and effect on light scattering distribution. In Advanced Microscopy Techniques (Vol. 7367). [73671J] https://doi.org/10.1117/12.831510

Study of 3D cell morphology and effect on light scattering distribution. / Ekpenyong, Andrew; Ding, Junhua; Yang, Li V.; Leffler, Nancy R.; Lu, Jun Q.; Brock, R. Scott; Hu, Xin Hua.

Advanced Microscopy Techniques. Vol. 7367 2009. 73671J.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ekpenyong, A, Ding, J, Yang, LV, Leffler, NR, Lu, JQ, Brock, RS & Hu, XH 2009, Study of 3D cell morphology and effect on light scattering distribution. in Advanced Microscopy Techniques. vol. 7367, 73671J, Advanced Microscopy Techniques, Munich, Germany, 6/14/09. https://doi.org/10.1117/12.831510
Ekpenyong A, Ding J, Yang LV, Leffler NR, Lu JQ, Brock RS et al. Study of 3D cell morphology and effect on light scattering distribution. In Advanced Microscopy Techniques. Vol. 7367. 2009. 73671J https://doi.org/10.1117/12.831510
Ekpenyong, Andrew ; Ding, Junhua ; Yang, Li V. ; Leffler, Nancy R. ; Lu, Jun Q. ; Brock, R. Scott ; Hu, Xin Hua. / Study of 3D cell morphology and effect on light scattering distribution. Advanced Microscopy Techniques. Vol. 7367 2009.
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