Study of 3D cell morphology and effect on light scattering distribution

Andrew E. Ekpenyong, Junhua Ding, Li V. Yang, Nancy R. Leffler, Jun Q. Lu, R. Scott Brock, Xin Hua Hu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.

Original languageEnglish (US)
Title of host publicationAdvanced Microscopy Techniques
DOIs
StatePublished - Oct 20 2009
Externally publishedYes
EventAdvanced Microscopy Techniques - Munich, Germany
Duration: Jun 14 2009Jun 16 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7367
ISSN (Print)1605-7422

Other

OtherAdvanced Microscopy Techniques
CountryGermany
CityMunich
Period6/14/096/16/09

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ekpenyong, A. E., Ding, J., Yang, L. V., Leffler, N. R., Lu, J. Q., Brock, R. S., & Hu, X. H. (2009). Study of 3D cell morphology and effect on light scattering distribution. In Advanced Microscopy Techniques [73671J] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7367). https://doi.org/10.1117/12.831510