Abstract
We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.
| Original language | English |
|---|---|
| Title of host publication | European Conference on Biomedical Optics, ECBO 2009 |
| Publisher | Optical Society of America |
| ISBN (Print) | 9780819476432 |
| State | Published - 2009 |
| Externally published | Yes |
| Event | European Conference on Biomedical Optics, ECBO 2009 - Munich, Germany Duration: Jun 14 2009 → Jun 18 2009 |
Other
| Other | European Conference on Biomedical Optics, ECBO 2009 |
|---|---|
| Country | Germany |
| City | Munich |
| Period | 6/14/09 → 6/18/09 |
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All Science Journal Classification (ASJC) codes
- Instrumentation
- Atomic and Molecular Physics, and Optics
Cite this
Study of 3D cell morphology and effect on light scattering distribution. / Ekpenyong, Andrew; Ding, Junhua; Yang, Li V.; Leffler, Nancy R.; Lu, Jun Q.; Scott Brock, R.; Hu, Xin Hua.
European Conference on Biomedical Optics, ECBO 2009. Optical Society of America, 2009.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Study of 3D cell morphology and effect on light scattering distribution
AU - Ekpenyong, Andrew
AU - Ding, Junhua
AU - Yang, Li V.
AU - Leffler, Nancy R.
AU - Lu, Jun Q.
AU - Scott Brock, R.
AU - Hu, Xin Hua
PY - 2009
Y1 - 2009
N2 - We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.
AB - We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.
UR - http://www.scopus.com/inward/record.url?scp=84897976695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897976695&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84897976695
SN - 9780819476432
BT - European Conference on Biomedical Optics, ECBO 2009
PB - Optical Society of America
ER -