Study of 3D cell morphology and effect on light scattering distribution

Andrew E. Ekpenyong; Junhua Ding; Li V. Yang; Nancy R. Leffler; Jun Q. Lu; R. Scott Brock; Xin Hua Hu

Study of 3D cell morphology and effect on light scattering distribution

Andrew E. Ekpenyong, Junhua Ding, Li V. Yang, Nancy R. Leffler, Jun Q. Lu, R. Scott Brock, Xin Hua Hu

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.

Original language	English (US)
Title of host publication	European Conference on Biomedical Optics, ECBO 2009
Publisher	Optical Society of America
ISBN (Print)	9780819476432
State	Published - Jan 1 2009
Externally published	Yes
Event	European Conference on Biomedical Optics, ECBO 2009 - Munich, Germany Duration: Jun 14 2009 → Jun 18 2009

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Other

Other	European Conference on Biomedical Optics, ECBO 2009
Country	Germany
City	Munich
Period	6/14/09 → 6/18/09

All Science Journal Classification (ASJC) codes

Instrumentation
Atomic and Molecular Physics, and Optics

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Cite this

Ekpenyong, A. E., Ding, J., Yang, L. V., Leffler, N. R., Lu, J. Q., Scott Brock, R., & Hu, X. H. (2009). Study of 3D cell morphology and effect on light scattering distribution. In European Conference on Biomedical Optics, ECBO 2009 (Optics InfoBase Conference Papers). Optical Society of America.

Study of 3D cell morphology and effect on light scattering distribution. / Ekpenyong, Andrew E.; Ding, Junhua; Yang, Li V.; Leffler, Nancy R.; Lu, Jun Q.; Scott Brock, R.; Hu, Xin Hua.

European Conference on Biomedical Optics, ECBO 2009. Optical Society of America, 2009. (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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abstract = "We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.",

author = "Ekpenyong, {Andrew E.} and Junhua Ding and Yang, {Li V.} and Leffler, {Nancy R.} and Lu, {Jun Q.} and {Scott Brock}, R. and Hu, {Xin Hua}",

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language = "English (US)",

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series = "Optics InfoBase Conference Papers",

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AU - Ding, Junhua

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AU - Leffler, Nancy R.

AU - Lu, Jun Q.

AU - Scott Brock, R.

AU - Hu, Xin Hua

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AB - We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.

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