Study of 3D cell morphology and effect on light scattering distribution

Andrew Ekpenyong, Junhua Ding, Li V. Yang, Nancy R. Leffler, Jun Q. Lu, R. Scott Brock, Xin Hua Hu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have acquired and reconstructed the 3D structures of B16F10 mouse melanoma cells to study morphology changes in response to gene variations. The 3D structure can be imported into a parallel FDTD code to model light scattering distribution and determine morphological parameters such as volumes of cytoplasm, nucleus and mitochondria. We found that the measured parameters agree with the light scatter data obtained with a flow cytometer, showing significant differences between the genetically modified and the unmodified melanoma cells.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2009
PublisherOptical Society of America
ISBN (Print)9780819476432
StatePublished - 2009
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2009 - Munich, Germany
Duration: Jun 14 2009Jun 18 2009

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2009
CountryGermany
CityMunich
Period6/14/096/18/09

Fingerprint

Mitochondria
Light scattering
light scattering
Genes
mitochondria
cytoplasm
cells
finite difference time domain method
genes
mice
nuclei

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Ekpenyong, A., Ding, J., Yang, L. V., Leffler, N. R., Lu, J. Q., Scott Brock, R., & Hu, X. H. (2009). Study of 3D cell morphology and effect on light scattering distribution. In European Conference on Biomedical Optics, ECBO 2009 Optical Society of America.

Study of 3D cell morphology and effect on light scattering distribution. / Ekpenyong, Andrew; Ding, Junhua; Yang, Li V.; Leffler, Nancy R.; Lu, Jun Q.; Scott Brock, R.; Hu, Xin Hua.

European Conference on Biomedical Optics, ECBO 2009. Optical Society of America, 2009.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ekpenyong, A, Ding, J, Yang, LV, Leffler, NR, Lu, JQ, Scott Brock, R & Hu, XH 2009, Study of 3D cell morphology and effect on light scattering distribution. in European Conference on Biomedical Optics, ECBO 2009. Optical Society of America, European Conference on Biomedical Optics, ECBO 2009, Munich, Germany, 6/14/09.
Ekpenyong A, Ding J, Yang LV, Leffler NR, Lu JQ, Scott Brock R et al. Study of 3D cell morphology and effect on light scattering distribution. In European Conference on Biomedical Optics, ECBO 2009. Optical Society of America. 2009
Ekpenyong, Andrew ; Ding, Junhua ; Yang, Li V. ; Leffler, Nancy R. ; Lu, Jun Q. ; Scott Brock, R. ; Hu, Xin Hua. / Study of 3D cell morphology and effect on light scattering distribution. European Conference on Biomedical Optics, ECBO 2009. Optical Society of America, 2009.
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