Subpopulations of Equine Infectious Anemia Virus Rev Coexist In Vivo and Differ in Phenotype

Prasith Baccam, Robert J. Thompson, Yuxing Li, Wendy O. Sparks, Michael A. Belshan, Karin S. Dorman, Yvonne Wannemuehler, J. Lindsay Oaks, James L. Cornette, Susan Carpenter

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Lentiviruses exist in vivo as a population of related, nonidentical genotypes, commonly referred to as quasispecies. The quasispecies structure is characteristic of complex adaptive systems and contributes to the high rate of evolution in lentiviruses that confounds efforts to develop effective vaccines and antiviral therapies. Here, we describe analyses of genetic data from longitudinal studies of genetic variation in a lentivirus regulatory protein, Rev, over the course of disease in ponies experimentally infected with equine infectious anemia virus. As observed with other lentivirus data, the Rev variants exhibited a quasispecies character. Phylogenetic and partition analyses suggested that the Rev quasispecies comprised two distinct subpopulations that coexisted during infection. One subpopulation appeared to accumulate changes in a linear, time-dependent manner, while the other evolved radially from a common variant. Over time, the two subpopulations cycled in predominance coincident with changes in the disease state, suggesting that the two groups differed in selective advantage. Transient expression assays indicated the two populations differed significantly in Rev nuclear export activity. Chimeric proviral clones containing Rev genotypes representative of each population differed in rate and overall level of virus replication in vitro. The coexistence of genetically distinct viral subpopulations that differ in phenotype provides great adaptability to environmental changes within the infected host. A quasispecies model with multiple subpopulations may provide additional insight into the nature of lentivirus reservoirs and the evolution of antigenic and drug-resistant variants.

Original languageEnglish
Pages (from-to)12122-12131
Number of pages10
JournalJournal of Virology
Volume77
Issue number22
DOIs
StatePublished - Nov 2003
Externally publishedYes

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Equine infectious anemia virus
Lentivirus
Phenotype
phenotype
rev Gene Products
Genotype
Population
Active Immunotherapy
Cell Nucleus Active Transport
genotype
regulatory proteins
Virus Replication
longitudinal studies
virus replication
disease course
Antiviral Agents
Longitudinal Studies
Clone Cells
clones
vaccines

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Baccam, P., Thompson, R. J., Li, Y., Sparks, W. O., Belshan, M. A., Dorman, K. S., ... Carpenter, S. (2003). Subpopulations of Equine Infectious Anemia Virus Rev Coexist In Vivo and Differ in Phenotype. Journal of Virology, 77(22), 12122-12131. https://doi.org/10.1128/JVI.77.22.12122-12131.2003

Subpopulations of Equine Infectious Anemia Virus Rev Coexist In Vivo and Differ in Phenotype. / Baccam, Prasith; Thompson, Robert J.; Li, Yuxing; Sparks, Wendy O.; Belshan, Michael A.; Dorman, Karin S.; Wannemuehler, Yvonne; Oaks, J. Lindsay; Cornette, James L.; Carpenter, Susan.

In: Journal of Virology, Vol. 77, No. 22, 11.2003, p. 12122-12131.

Research output: Contribution to journalArticle

Baccam, P, Thompson, RJ, Li, Y, Sparks, WO, Belshan, MA, Dorman, KS, Wannemuehler, Y, Oaks, JL, Cornette, JL & Carpenter, S 2003, 'Subpopulations of Equine Infectious Anemia Virus Rev Coexist In Vivo and Differ in Phenotype', Journal of Virology, vol. 77, no. 22, pp. 12122-12131. https://doi.org/10.1128/JVI.77.22.12122-12131.2003
Baccam, Prasith ; Thompson, Robert J. ; Li, Yuxing ; Sparks, Wendy O. ; Belshan, Michael A. ; Dorman, Karin S. ; Wannemuehler, Yvonne ; Oaks, J. Lindsay ; Cornette, James L. ; Carpenter, Susan. / Subpopulations of Equine Infectious Anemia Virus Rev Coexist In Vivo and Differ in Phenotype. In: Journal of Virology. 2003 ; Vol. 77, No. 22. pp. 12122-12131.
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