Sulfation of iodothyronines by recombinant human liver steroid sulfotransferases

Xinying Li, Robert J. Anderson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Sulfation is an important pathway in the metabolism of thyroid hormones. Sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal human fetal circulation. We assayed and characterized COS-1 cell expressed recombinant human liver dehydroepiandrosterone sulfotransferase (DHEA ST or SULT2A1) and estrogen sulfotransferase (EST or SULT1E1) activities for the first time with triiodothyronine (T3) as the substrate. Several biochemical properties that included apparent K(m) values, thermal stabilities, and responses to the inhibitors 2,6-dichloro-4-nitrophenol and NaCl were tested. SULT2A1, a member of the hydroxysteroid sulfotransferase family, used 3,3'-T2 more readily than T3 and 3,5-T2 as substrates, but had the lowest apparent K(m) value for T3 of any reported human SULT. SULT1E1, a member of the phenol sulfotransferase family, used 3,3'-T2 and rT3 more readily than T3, and also displayed the greatest specificity for T4 among human SULTs. SULT2A1 may contribute more to iodothyronine sulfation than previously suspected. Potential roles of both steroid sulfotransferases in the enhanced sulfation of nonthyroidal illnesses and fetal development invite further investigation.

Original languageEnglish
Pages (from-to)632-639
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume263
Issue number3
DOIs
StatePublished - Oct 5 1999

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Liver
Arylsulfotransferase
Dehydroepiandrosterone
Expressed Sequence Tags
Triiodothyronine
Substrates
Thyroid Hormones
Metabolism
Thermodynamic stability
COS Cells
Fetal Development
Hot Temperature
3,3'-diiodothyronine
steroid sulfotransferase
3,5-diiodothyronine
estrone sulfotransferase
dehydroepiandrosterone sulfotransferase
alcohol sulfotransferase
2,6-dichloro-4-nitrophenol

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sulfation of iodothyronines by recombinant human liver steroid sulfotransferases. / Li, Xinying; Anderson, Robert J.

In: Biochemical and Biophysical Research Communications, Vol. 263, No. 3, 05.10.1999, p. 632-639.

Research output: Contribution to journalArticle

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